Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CTLA‑4 Fc Chimera is coated at 0.5 µg/mL, 100 μL/well, Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) binds with an ED50 of 5-30 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Cynomolgus Monkey CTLA-4
    (Ala37-Asp161)
    Accession # XP_005574071
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala37
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    40 kDa
  • SDS-PAGE
    46-57 kDa, reducing conditions
9336-CT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Cynomolgus Monkey CTLA‑4 FcChimera (Catalog # 9336-CT) is coated at 0.5 µg/mL,100 μL/well, Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) binds with anED50 of 5-30 ng/mL.
Background: CTLA-4
CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Cyno CTLA-4 has a 98% homology to human CTLA-4. Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41-43 kDa (2). Within the ECD, human CTLA-4 shares 98%, 68%, 71% and 83-86% aa sequence identity with cynomolgus, mouse, rat and porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3-5). In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1-2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11-13).
  • References:
    1. Harper, K. et al. (1991) J. Immunol. 147:1037.
    2. Teft, W.A. et al. (2006) Annu. Rev. Immunol. 24:65.
    3. Magistrelli, G. et al. (1999) Eur. J. Immunol. 29:3596.
    4. Tector, M. et al. (2009) BMC Immunol. 10:51.
    5. Oaks, M.K. and K.M. Hallett (2000) J. Immunol. 164:5015.
    6. Vijayakrishnan, L. et al. (2004) Immunity 20:563.
    7. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
    8. Jansson, A. et al. (2005) J. Immunol 175:1575.
    9. Darlington, P.J. et al. (2005) J. Immunol. 175:996.
    10. Platt, A.M. et al. (2010) J. Immunol. 185:1558.
    11. Wing, K. et al. (2008) Science. 322: 271.
    12. Friedline, R.H. et al. (2009) J. Exp. Med. 206:421.
    13. Jain, N. et al. (2010) Proc. Natl. Acad. Sci. USA. 107:1524.
  • Long Name:
    Cytotoxic T-lymphocyte-associated Molecule 4
  • Entrez Gene IDs:
    1493 (Human); 12477 (Mouse); 102115124 (Cynomolgus Monkey)
  • Alternate Names:
    CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
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