>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Fc gamma RIIA/CD32a is immobilized on
a His Tag Antibody coated plate, it binds Biotinylated Human IgG. The
of Biotinylated Human IgG that
produces 50% of the optimal binding response is approximately 0.75-3.75
Human embryonic kidney cell, HEK293-derived Thr29-Ile211, with a C-terminal 6-His tag
When Recombinant CynomolgusMonkey Fc gamma RIIA/CD32a (Catalog # 9240-CD) is immobilized on a His Tag Antibodycoated plate, Biotinylated Human IgG binds with an ED50 of 0.75-3.75 μg/mL.
Background: Fc gamma RIIA/CD32a
Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1, 2). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. CD32 proteins are encoded by three genes in human (Fc gamma RII A, B, and C), two in cynomolgus (Fc gamma RII A and B), and one in mouse (Fc gamma RIIB). Fc gamma RII A and C contain ITAM sequences, while Fc gamma RIIB contains an ITIM sequence in their cytoplasmic domains. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1, 3). Mature cynomolgus Fc gamma RIIA consists of a 183 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 77 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RIIA shares 89% aa sequence identity with human Fc gamma RIIA. It binds cynomolgus and human IgG subclasses 1-4 as well as human Pentraxin-2, and C-Reactive Protein (4, 5). It delivers activating signals through its cytoplasmic ITAM sequence (6). Fc gamma RIIA signaling cooperates with Integrin alpha 2b beta 3 and the CD42b-GP1b-IX-V complex to promote platelet adhesion and activation (7, 8). Fc gamma RIIA is expressed on monocytes, monocytes, and platelets and additionally on cynomolgus granulocytes (1, 4).
Qiao, J. et al. (2015) Immunol. Rev. 268:241.
Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
Takai, T. (2002) Nature Rev. Immunol. 2:580.
Warncke, M. et al. (2012) J. Immunol. 88:4405.
Lu, J. et al. (2008) Nature 456:989.
Rosenfeld, S.I. et al. (1985) J. Clin. Invest. 76:2317.
Sullam, P.M. et al. (1998) J. Biol. Chem. 273:5331.
CD32 antigen; CD32a; CD32MGC23887; CDw32fc-gamma-RIIa; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32); Fc gamma RIIA; FCG2; Fc-gamma RII-a; Fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; IGFR2MGC30032; IgG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.