Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF

Catalog # Availability Size / Price Qty
140-B1-100
140-B1-01M
Product Details
Citations (9)
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Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.025‑0.15 µg/mL in the presence of PHA.
Source
Mouse myeloma cell line, NS0-derived human B7-1/CD80 protein
Human B7-1
(Val35-Asn242)
Accession # NP_005182
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val35
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.3 kDa (monomer)
SDS-PAGE
70-90 kDa, reducing conditions

Product Datasheets

140-B1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature human B7-1 consists of a 208 aa extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain (7). Within the ECD, human B7-1 shares 50% aa sequence identity with mouse and rat
B7-1. Alternative splicing generates a 30 kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and
CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2).

References
  1. Ville, S. et al. (2015) Front. Immunol. 6:411.
  2. Azuma, M. et al. (1993) Nature 366:76.
  3. Freeman, G.J. et al. (1993) Science 262:909.
  4. Chen, C. et al. (1994) J. Immunol. 152:4929.
  5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  6. Lanier, L. et al. (1995) J. Immunol. 154:97.
  7. Freeman, G.J. et al. (1989) J. Immunol. 143:2714.
  8. Kakoulidou, M. et al. (2007) Scand. J. Immunol. 66:529.
Entrez Gene IDs
941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

Citations for Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells
    Authors: BD Hock, SA MacPherson, JL McKenzie
    PLoS ONE, 2017;12(3):e0172858.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A CD80-Biased CTLA4-Ig Fusion Protein with Superior In Vivo Efficacy by Simultaneous Engineering of Affinity, Selectivity, Stability, and FcRn Binding
    Authors: Lutz Jermutus
    J. Immunol., 2016;0(0):.
    Applications: Bioassay
  3. Genomic landscape of cutaneous T cell lymphoma.
    Authors: Choi J, Goh G, Walradt T, Hong B, Bunick C, Chen K, Bjornson R, Maman Y, Wang T, Tordoff J, Carlson K, Overton J, Liu K, Lewis J, Devine L, Barbarotta L, Foss F, Subtil A, Vonderheid E, Edelson R, Schatz D, Boggon T, Girardi M, Lifton R
    Nat Genet, 2015;47(9):1011-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. Establishment of long-term tolerance to SRBC in dogs by recombinant canine CTLA4-Ig.
    Authors: Graves SS, Stone D, Loretz C, Peterson L, McCune JS, Mielcarek M, Storb R
    Transplantation, 2009;88(3):317-22.
    Species: Canine
    Sample Types: Serum
    Applications: ELISA capture
  5. Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins.
    Authors: Kerr SC, Fieger CB, Snapp KR, Rosen SD
    J. Immunol., 2008;181(2):1480-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A.
    Authors: Narazaki M, Segarra M, Tosato G
    Blood, 2008;111(8):4126-36.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ctrl
  7. The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm.
    Authors: Sakthivel P, Shively V, Kakoulidou M
    J. Intern. Med., 2007;261(4):399-407.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  8. ICOS cooperates with CD28, IL-2, and IFN-gamma and modulates activation of human naive CD4+ T cells.
    Authors: Mesturini R, Nicola S, Chiocchetti A, Bernardone IS, Castelli L, Bensi T, Ferretti M, Comi C, Dong C, Rojo JM, Yagi J, Dianzani U
    Eur. J. Immunol., 2006;36(10):2601-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A.
    Authors: Narazaki M, Tosato G
    Blood, 2006;107(10):3892-901.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard

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