Recombinant Human BTN2A2/Butyrophilin 2A2 Protein Fc, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.1-0.5 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Human BTN2A2
    (Gln33-Val237)
    Accession # Q8WVV5
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminusC-terminus
  • N-terminal Sequence
    Analysis
    No result obtained. Gln33 inferred from enzymatic pyroglutamate treatment revealing Phe34
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    50 kDa
8918-BT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human BTN2A2/Butyrophilin 2A2 Fc Chimera (Catalog # 8918-BT) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.1-0.5 μg/mL.
Background: BTN2A2/Butyrophilin 2A2
Butyrophilin 2A2 (BTN2A2) is a widely expressed 65 kDa type I transmembrane glycoprotein that functions as a negative regulator of immune responses (1). Mature human Butyrophilin 2A2 consisits of a 233 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 237 aa cytoplasmic domain. Within the Ig-like domains, human Butyrophilin 2A2 shares 70% aa sequence identity with mouse and rat Butyrophilin 2A2. Alternative splicing generates additional isoforms of human Butyrophilin 2A2 that lack the first, second, or both Ig-like domains as well as isoforms with substitutions and deletions in the cytoplasmic region. Within the immune system, Butyrophilin 2A2 is expressed on thymic epithelial cells, naïve B cells, splenic NK cells, dendritic cells, and peritoneal macrophages and is up-regulated with cell activation (2, 3). Butyrophilin 2A2 inhibits T cell proliferation and activation and enhances the development of FoxP3+ regulatory T cells (2, 3). Its up-regulation in the hippocampus is associated with schizophrenia (4).
  • References:
    1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
    2. Smith, I.A. et al. (2010) J. Immunol. 184:3514.
    3. Ammann, J.U. et al. (2013) J. Immunol. 190:5030.
    4. Sinkus, M.L. et al. (2013) Brain Behav. Immun. 32:51.
  • Long Name:
    Butyrophilin Subfamily 2 Member A2
  • Entrez Gene IDs:
    10385 (Human); 238555 (Mouse); 306957 (Rat)
  • Alternate Names:
    BTF2; BTF2BT2.2butyrophilin 2; BTN2; BTN2A2; Butyrophilin 2; butyrophilin subfamily 2 member A2; butyrophilin, subfamily 2, member A2; FLJ41908
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