Recombinant Human C1qTNF10 Protein, CF

  
  • Purity
    >70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human C1qTNF10/C1qL2 is immobilized at 1 μg/mL, 100 μL/well, the concentration of recombinant human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 0.075-0.45 μg/mL.
  • Source
    E. coli-derived Phe154-Asp287, with an N-terminal Met and 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    16 kDa
  • SDS-PAGE
    14 kDa, reducing conditions
9189-TN
 
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP and Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human C1qTNF10 (Catalog #
9189-TN)is coated at 1 μg/mL, 100 μL/well, Recombinant Human BAI2 Fc Chimera binds with a ED50 of
0.075-0.45 μg/mL.
Background: C1qTNF10/C1qL2
C1qTNF10 (CTRP10), also known as C1qL2, is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Mature human C1qTNF10 contains a collagen-like region and one C1q-like domain. It can form heterotrimers and higher order disulfide linked oligomers with C1qTNF1, C1qTNF11/C1qL4, and C1qTNF13/C1qL3 (3-6). Within the C1q-like domain, human C1qTNF10 shares 100% aa sequence identity with mouse and rat C1qTNF10. Similarly to C1qTNF11, C1qTNF13, and C1qTNF14/C1qL1, C1qTNF10 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (7). It is produced by mossy fibers in the hippocampus where it enhances the postsynaptic clustering of kainate-type glutamate receptors (KAR) on CA3 pyramidal neurons (8). This function is enhanced by the binding of C1qTNF10 to the KAR subunits GluK2 and GluK4 as well as to Neurexin-3 beta containing the exon 5b splice site insertion (8).
  • References:
    1. Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
    2. Yuzaki, M. (2001) Eur. J. Neurosci. 32:191.
    3. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
    4. Wei, Z. et al. (2011) J. Biol. Chem. 286:15652.
    5. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
    6. Wong, G.W. et al. (2008) Biochem. J. 416:161.
    7. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
    8. Matsuda, K. et al. (2016) Neuron 90:752.
  • Long Name:
    C1q And Tumor Necrosis Factor Related Protein 10
  • Entrez Gene IDs:
    165257 (Human); 226359 (Mouse)
  • Alternate Names:
    C1q and tumor necrosis factor related protein 10; C1q-domain containing protein; C1qL2; C1QTNF10; C1qTNF10/C1qL2; complement C1q-like protein 2; complement component 1, q subcomponent-like 2; CTRP10
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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