Recombinant Human CD28H/IGPR-1 His-tag Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human CD28H/IGPR-1 is immobilized at 0.125 μg/mL, 100 μL/well, the concentration of Recombinant Human
    B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) that produces 50% of the optimal binding response is approximately 0.2-1 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Leu23-Gly150, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu23
  • Predicted Molecular Mass
    15 kDa
  • SDS-PAGE
    21-34 kDa, reducing conditions
9236-TR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human CD28H/IGPR-1 (Catalog # 9236-TR) isimmobilized at 0.125 μg/mL, Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) binds with an ED50 of
0.2-1 μg/mL.
Background: CD28H/IGPR-1
CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).
  • References:
    1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
    2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
    3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
    4. Huang, C. et al. (2003) Nature 424:219.
    5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.
  • Long Name:
    Transmembrane And Immunoglobulin Domain Containing 2
  • Entrez Gene IDs:
    126259 (Human)
  • Alternate Names:
    CD28H/IGPR-1; IGPR1; IGPR-1; TMIGD2
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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