Recombinant Human Cerebellin-2 Protein, CF
Recombinant Human Cerebellin-2 Protein, CF Summary
Arg50-Leu224, with an N-terminal HA (YPYDVPDYA) tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cerebellin-2 (CBLN2) is an approximately 25 kDa N-glycosylated protein in the Cerebellin family of TNF superfamily molecules (1). Cerebellins contain a collagenous domain and a C-terminal TNF/C1q-like domain. Human Cerebellin-2 consists of this 175 amino acid (aa) region following an N-terminal 28 aa potential cytoplasmic domain and a 21 aa potential transmembrane segment. Within the Cerebellin-like region, human Cerebellin-2 shares 99% aa sequence identity with mouse, rat, and chick Cerebellin-2 (2‑4). It is expressed in multiple regions of the nervous system including the neocortex, olfactory bulb, thalamus, hypothalamus, midbrain (5), preganglionic sensory nerves, dorsal root ganglia, and sensory and motoneurons of the spinal cord (4, 6) as well as in the zona glomerulosa of the adrenal cortex (7). Cerebellin-2 forms cell associated homo-oligomers and heteromers with Cerebellins-1, -3, and -4 [Iijima 1049]. It binds with high affinity to alpha and beta Neurexin 1 isoforms that contain the splice site 4 insert (+SS4) and more weakly to Neurexin 2 beta (+SS4) and Neurexin 3 beta (+SS4) (9, 10). These interactions promote presynaptic differentiation in cultures of cortical and cerebellar neurons (9, 10).
- Yuzaki, M. (2011) Curr. Opin. Neurobiol. 21:215.
- Kavety, B. et al. (1994) Brain Res. Mol. Brain Res. 27:152.
- Wada, C. and H. Ohtani (1991) Brain Res. Mol. Brain Res. 9:71.
- Yang, M. et al. (2010) J. Comp. Neurol. 518:2818.
- Miura, E. et al. (2006) Eur. J. Neurosci. 24:750.
- Reiner, A. et al. (2011) J. Comp. Neurol. 519:2225.
- Rucinski, M. et al. (2005) Int. J. Mol. Med. 15:411.
- Iijima, T. et al. (2007) Eur. J. Neurosci. 25:1049.
- Joo, J.-Y. et al. (2011) Biochem. Biophys. Res. Commun. 406:627.
- Matsuda, K. and M. Yuzaki (2011) Eur. J. Neurosci. 33:1447.
Citation for Recombinant Human Cerebellin-2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Defining the ligand specificity of the deleted in colorectal cancer (DCC) receptor.
Authors: Haddick P, Tom I, Luis E, Quinones G, Wranik B, Ramani S, Stephan J, Tessier-Lavigne M, Gonzalez L
PLoS ONE, 2014;9(1):e84823.
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
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