Recombinant Human CUL1/NEDD8/RBX1 Complex, CF

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Product Details

Recombinant Human CUL1/NEDD8/RBX1 Complex, CF Summary

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL1/NEDD8/RBX1 Complex protein
Predicted Molecular Mass
105 kDa (neddylated CUL1), 12 kDa (RBX1)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: CUL1/NEDD8/RBX1 Complex

Cullin-1 (CUL1) is a core component of multiple SCF (SKP1-CUL1-F-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  In the SCF complex, CUL1 serves as a scaffold that organizes the SKP1-F-box recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  This complex consists of an N-terminal 10-His tagged CUL1 and an untagged RBX1. CUL1 is neddylated in this complex.

  1. Baek K., et al. (2020) Nature  578: 461
  2. Duda D.M., et al.  (2008) Cell  134: 995
  3. Duda D.M., et al.  (2012) Mol. Cell  47: 371
  4. Goldenberg S.J., et al.  (2004) Cell  119: 517
  5. Zheng N., et al.  (2002) Nature  416: 703
Long Name
Cullin-1/Neddylin/RING Box Protein 1 Complex
Entrez Gene IDs
8454 (Human)
Alternate Names
CUL1/NEDD8/RBX1 Complex


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