Recombinant Human CUL4A/NEDD8/RBX1 Complex Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: CUL4A/NEDD8/RBX1 Complex
Cullin-4A (CUL4A) is a core component of multiple cullin-RING type E3
Ubiquitin ligase complexes that mediate the ubiquitination of proteins
involved in cell cycle progression, DNA repair and other processes. In
the DCX complex (DDB1-CUL4-X-box) CUL4A serves as a scaffold that
organizes the DDB1-X-box recognition subunits with the RBX1 subunit and
contributes to catalysis through positioning of the substrate and an E2
ubiquitin-conjugating enzyme. In vivo, the E3 ubiquitin ligase activity
of the DCX complex is dependent on neddylation of the cullin subunit,
though neddylation may be dispensable for some in vitro reactions. This
complex consists of an N-terminal 10-His tagged CUL4A and an untagged
RBX1. CUL4A is neddylated in this complex.
- Baek K., et al. (2020) Nature 578: 461
- Fischer E.S., et al. (2014) Nature 512: 49
- He Y.J., et al. (2006) Genes Dev. 20: 2949
- Ito T., et al. (2010) Science 327: 1345
- Wang H., et al. (2006) Mol. Cell 22: 383
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