Recombinant Human CUL4A/NEDD8/RBX1 Complex Protein, CF

Catalog # Availability Size / Price Qty
E3-441-025
Product Details
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Recombinant Human CUL4A/NEDD8/RBX1 Complex Protein, CF Summary

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL4A/NEDD8/RBX1 Complex protein
Predicted Molecular Mass
104 kDa (neddylated CUL4A), 12 kDa (RBX1)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-441

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CUL4A/NEDD8/RBX1 Complex

Cullin-4A (CUL4A) is a core component of multiple cullin-RING type E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes.  In the DCX complex (DDB1-CUL4-X-box) CUL4A serves as a scaffold that organizes the DDB1-X-box recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the DCX complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  This complex consists of an N-terminal 10-His tagged CUL4A and an untagged RBX1. CUL4A is neddylated in this complex.

References
  1. Baek K., et al. (2020) Nature 578: 461
  2. Fischer E.S., et al.  (2014) Nature  512: 49
  3. He Y.J., et al.  (2006) Genes Dev.  20: 2949
  4. Ito T., et al.  (2010) Science  327: 1345
  5. Wang H., et al.  (2006) Mol. Cell  22: 383
Long Name
Cullin-4A/Neddylin/RING Box Protein 1 Complex
Entrez Gene IDs
8451 (Human)
Alternate Names
CUL4A/NEDD8/RBX1 Complex

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