Recombinant Human Fc mu R/FAIM3 Protein, CF

Catalog # Availability Size / Price Qty
9494-MU-050
Recombinant Human Fc mu R/FAIM3 Protein Bioactivity
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Citations (1)
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Recombinant Human Fc mu R/FAIM3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When human IgM is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Fc mu R/FAIM3 binds with an ED50 of 0.4-2 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Fc mu R/FAIM3 protein
Human Fc mu R/FAIM3
(Arg18-Gly251)
Accession # O60667-1
HisHis HA
(YPYDVPDYA)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Arg18
Predicted Molecular Mass
27 kDa
SDS-PAGE
39-50 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9494-MU

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Fc mu R/FAIM3 Protein Bioactivity View Larger

When human IgM is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Fc mu R/FAIM3 (Catalog # 9494-MU) binds with an ED50 of 0.4-2 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc mu R/FAIM3

The human FAIM3 gene (also known as FCMR or TOSO), is a transmembrane sialoglycoprotein expressed mainly by lymphocytes. FAIM3 is a type I membrane protein with an intracellular C-terminal domain and an extracellular N-terminal domain (1). The extracellular domain has homology to the immunoglobulin variable region domains (1) and FAIM3 is identified as an Fc receptor for IgM (2, 3). The amino acid sequence of human FAIM3 is 58% and 55% identical to that of mouse and rat FAIM3, respectively. FAIM3 was shown to be over-expressed in chronic lymphocytic leukemia (CLL) (4) and associated with disease progression (5, 6). FAIM3 has also been linked to the homeostasis and activation of the innate immune system (7). Interestingly, there is growing evidence that neurodegenerative diseases are associated with the activation of the immune surveillance system. This system is responsible for controlling danger signals and responding accordingly to the magnitude and duration of the threat (8, 9).

References
  1. Song, Y. et al (2005) J. Biol. Chem. 280(10):9618.
  2. Shima H et al. (2010) Int. Immunol. 22:149.
  3. Kubagawa H et al. (2009) J. Exp. Med. 206:2779.
  4. Proto-Siqueira R et al. (2008) Blood, 112:394.
  5. Pallasch, C et al. (2008) Blood, 112:4213.
  6. Pallasch, C P et al (2009) Leukemia & lymphoma, 50:498.
  7. Sigruener, A et al (2007) Biochemical and biophysical research communications, 359:723.
  8. Richards R I et al (2016) Front. Neurosci. 10:193.
  9. Planells-Ferrer L et al (2016) J Neurochem. 139:11.
Long Name
Fc mu Receptor
Entrez Gene IDs
9214 (Human); 69169 (Mouse); 548326 (Rat)
Alternate Names
FAIM3; Fas apoptotic inhibitory molecule 3; Fc mu R; Fc mu receptor; FCMR; immunoglobulin mu Fc receptor; Regulator of Fas-induced apoptosis Toso; Toso; TOSOIgM Fc receptor

Citation for Recombinant Human Fc mu R/FAIM3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Diverse Functional Autoantibodies in Patients with COVID-19
    Authors: EY Wang, T Mao, J Klein, Y Dai, JD Huck, F Liu, NS Zheng, T Zhou, B Israelow, P Wong, C Lucas, J Silva, JE Oh, E Song, ES Perotti, S Fischer, M Campbell, JB Fournier, AL Wyllie, CBF Vogels, IM Ott, CC Kalinich, ME Petrone, AE Watkins, CD Cruz, SF Farhadian, WL Schulz, ND Grubaugh, AI Ko, A Iwasaki, AM Ring
    medRxiv : the preprint server for health sciences, 2020;0(0):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Capture

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