Recombinant Human FGF basic (146 aa) GMP Protein, CF

Animal-Free.
  
  • Purity
    >97%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.
    The specific activity of Recombinant Human FGF basic GMP is approximately 800 IU/μg, which is calibrated against human FGF basic WHO International Standard (NIBSC code: 90/712).
  • Source
    E. coli-derived Pro143-Ser288, with an N-terminal Ala Produced using non-animal reagents in an animal-free laboratory.
    Manufactured and tested under cGMP guidelines.
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala-Pro143-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser
  • Predicted Molecular Mass
    16.5 kDa
  • SDS-PAGE
    17 kDa, reducing conditions
233-GMP
 
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • A minimum of 6 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Data Images
GMP‑grade Recombinant Human FGF basic (Catalog # 233-GMP) stimulates proliferation of the NR6R‑3T3 mouse fibro­blast cell line. The ED50 is
0.1-0.6 ng/mL.
1 µg/lane of GMP-grade Recombinant Human FGF basic (Catalog # 233-GMP) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a single band at 17 kDa.
Mass Spectrometry
MALDI‑TOF analysis of GMP‑grade Recombinant Human FGF basic (Catalog # 233-GMP). The major peak corresponds to the calculated molecular mass, 16479 Da. The minor peak at 16691 Da is a matrix-associated artifact of the MALDI-TOF.
Background: FGF basic

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

  • References:
    1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
    2. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
    3. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
    4. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.
  • Long Name:
    Fibroblast Growth Factor basic
  • Entrez Gene IDs:
    2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
  • Alternate Names:
    basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin

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