Recombinant Human FGF basic (157 aa) Protein, CF

(7 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.5-2.5 ng/mL.
  • Source
    E. coli-derived Gly132-Ser288
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly132
  • Predicted Molecular Mass
    17.4 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
234-FSE/CF
 
234-FSE
Formulation Supplied as a 0.2 μm filtered solution in Tris-HCl and NaCl.
Formulation Supplied as a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Data Images
Recombinant Human FGF basic (157 aa) (Catalog # 234-FSE/CF) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.5-2.5 ng/mL.
1 μg/lane of Recombinant Human FGF basic (157 aa) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Background: FGF basic

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35 - 60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

  • References:
    1. Coulier, F. et al., 1997, J. Mol. Evol. 44:43.
    2. Chen, C.H. et al., 2004, Curr. Vasc. Pharmacol. 2:33.
    3. Mohammadi, M. et al., 2005, Curr. Opin. Struct. Biol. 15:506.
    4. Fernig, D. et al., 1994, Prog. Growth Factor Res. 5:353.
  • Long Name:
    Fibroblast Growth Factor basic
  • Entrez Gene IDs:
    2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
  • Alternate Names:
    basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. A Combination of Culture Conditions and Gene Expression Analysis Can Be Used to Investigate and Predict hES Cell Differentiation Potential towards Male Gonadal Cells.
    Authors: Kjartansdottir K, Reda A, Panula S, Day K, Hultenby K, Soder O, Hovatta O, Stukenborg J
    PLoS ONE, 2015;10(12):e0144029.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Antifibrotic and anti-inflammatory activity of the tyrosine kinase inhibitor nintedanib in experimental models of lung fibrosis.
    Authors: Wollin L, Maillet I, Quesniaux V, Holweg A, Ryffel B
    J Pharmacol Exp Ther, 2014;349(2):209-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Interactions between important regulatory proteins and human alphaB crystallin.
    Authors: Ghosh JG, Shenoy AK, Clark JI
    Biochemistry, 2007;46(21):6308-17.
    Species: Human
    Sample Type: Peptide
    Application: ELISA-based Protein Pin Array
  5. Neuritogenic activity of chondroitin/dermatan sulfate hybrid chains of embryonic pig brain and their mimicry from shark liver. Involvement of the pleiotrophin and hepatocyte growth factor signaling pathways.
    Authors: Li F, Shetty AK, Sugahara K
    J. Biol. Chem., 2007;282(5):2956-66.
    Species: Fish - Prionace glauca (blue shark)
    Sample Type: Peptide
    Application: Bioassay
  6. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006;108(3):928-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006;169(4):1167-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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