Recombinant Human FLAG-NEDD8 Vinyl Sulfone Protein, CF

Catalog # Availability Size / Price Qty
UL-803-025
Product Details
Citations (3)
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Recombinant Human FLAG-NEDD8 Vinyl Sulfone Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Add Recombinant Human FLAG-NEDD8 Vinyl Sulfone to in vitro assays to inhibit NEDD8-specific isopeptidases. The FLAG-tag allows for detection and purification of NEDD8-specific isopeptidases activity. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human FLAG-NEDD8 Vinyl Sulfone concentration of 1-5 μM.
Source
E. coli-derived human NEDD8 protein
Contains an N‑terminal FLAG (DYKDDDDK) tag and a C‑terminal Vinyl Sulfone
Accession #
Predicted Molecular Mass
9.8 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-803

Formulation

X mg/ml (X μM) in 20 mM HEPES pH 7.5, 100 mM NaCl

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: NEDD8

Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one Ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide (1,2). Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat, and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits (3). Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes (4). Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates (5). For example, transfer of NEDD8 to the Cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of Ubiquitin ligases (E3) (6-8). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival (9,10). Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies (11,12).

Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Entrez Gene IDs
4738 (Human); 18002 (Mouse); 25490 (Rat)
Alternate Names
FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8

Citations for Recombinant Human FLAG-NEDD8 Vinyl Sulfone Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Nedd8 hydrolysis by UCH proteases in Plasmodium parasites
    Authors: M Karpiyevic, S Adjalley, M Mol, DB Ascher, B Mason, GJ van der He, H Laman, H Ovaa, MCS Lee, K Artavanis-
    PLoS Pathog., 2019;15(10):e1008086.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Nedd8 hydrolysis by UCH proteases in Plasmodium parasites
    Authors: M Karpiyevic, S Adjalley, M Mol, DB Ascher, B Mason, GJ van der He, H Laman, H Ovaa, MCS Lee, K Artavanis-
    PLoS Pathog., 2019;15(10):e1008086.
    Species: Parasite - Plasmodium falciparum
    Sample Types: Whole Organism
  3. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells.
    Authors: Gastaldello S, Chen X, Callegari S, Masucci M
    PLoS Pathog, 2013;9(10):e1003664.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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