Recombinant Human GPR158 His-tag Protein, CF Summary
Ala24-Gln411, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 1 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human GPR158 (Catalog # 10286-GP) is coated at 1 µg/mL, 100 µL/well, Recombinant Human Neuropilin-1 (Catalog # 3870-N1) binds with an ED50 of 0.8-4 µg/mL.
2 μg/lane of Recombinant Human GPR158 His-tag Protein (Catalog # 10286-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.
G-protein coupled receptor 158 (GPR158) is a receptor belonging to the Class C GPCR family. It lacks the extracellular Venus flytrap module characteristic of the known members of that family and instead contains two other elements that are not typical of the class: a calcium-binding EGF-like domain and a leucine repeat region (1, 2). The mature extracellular domain of human GPR158 contains 393 amino acids (aa) and shares 89% identity with both mouse and rat GPR158. GPR158 is expressed at the highest level in the brain, but also in a variety of other tissues including retina, spleen, liver and lung (3). GPR158 was originally identified in functional screens linked with biological stress and has been implicated in the osteocalcin effect on cognitive processes in the brain (4, 5), and glaucoma and cancer in the periphery (4, 6).
- Jingami, H. et al. (2003) Curr. Opin. Neurobiol. 13:271.
- Bjarnadóttir, T.K et al. (2005) Gene. 362:70.
- Orlandi, C. et al. (2012) J. Cell Biol. 197:711.
- Itakura, T. et al. (2019) J. Ocul. Pharmacol. Ther. 35:203.
- Khrimian, L. et al. (2017) J. Exp. Med. 214:2859.
- Fenner, A. (2015) Nat. Rev. Urol. 12:182.
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