Recombinant Human IL-11 GMP Protein, CF

Animal Component Free Process.
  
  • Purity
    >95%, by SDS-PAGE with silver staining, under reducing conditions.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.12 ng/mL.
    The specific activity of recombinant human IL-11 is approximately 1.05 x 104 units/μg, which is calibrated against recombinant human IL-11 WHO Standard (NIBSC code: 92/788).
  • Source
    Spodoptera frugiperda, Sf 9 (baculovirus)-derived Pro22-Leu199 Produced in an animal component free process (ACFP).
    Manufactured and tested under cGMP guidelines.
  • Accession #
  • N-terminal Sequence
    Analysis
    Pro-Gly-Pro-Pro-Pro-Gly-Pro-Pro-Arg-Val
  • Predicted Molecular Mass
    19 kDa
  • SDS-PAGE
    20 kDa, reducing conditions
218-GMP
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • A minimum of 6 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Data Images

GMP-grade Recombinant Human IL-11 (Catalog # 218-GMP) stimulates cell proliferation of the T11 mouse plasmacytoma cell line. The ED50 for this effect is
0.02-0.12 ng/mL.

1 μg/lane of Recombinant Human GMP-grade IL-11 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.

Background: IL-11

IL-11 (Interleukin 11) is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27 and IL-31 (1-3). In humans, IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF) (3). The human IL-11 cDNA encodes a 199 amino acid (aa) precursor, which generates a 178 aa, 19 kDa mature unglycosylated protein. Mature human IL-11 shares 88%, 88%, and 96% aa sequence identity with mouse, rat and canine IL-11, respectively. IL-11 is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts, among other cell types (1). It is found in the plasma mainly during inflammation, such as that associated with viral infection, cancer, or inflammatory arthritis, and is considered to be primarily anti‑inflammatory (1). It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine (1). It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis (1). IL-11 synergizes with several other cytokines to produce these effects, and its effects overlap with those of IL-6 (1). IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R alpha subunit and two molecules of the ubiquitously expressed cell signaling beta subunit, gp130 (4). A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface, or inhibit cell surface IL-11 R alpha /gp130 signaling (5-7).

  • References:
    1. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
    2. Paul, S.R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7512.
    3. Kawashima, I. et al. (1991) FEBS Lett. 283:199.
    4. Barton, V.A. et al. (2000) J. Biol. Chem. 275:36197.
    5. Curtis, D.J. et al. (1997) Blood 90:4403.
    6. Baumann, H. et al. (1996) J. Immunol. 157:284.
    7. Karow, J. et al. (1996) Biochem. J. 318:489.
  • Long Name:
    Interleukin 11
  • Entrez Gene IDs:
    3589 (Human); 16156 (Mouse); 171040 (Rat)
  • Alternate Names:
    Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11

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