Recombinant Human Intelectin-1/Omentin, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Streptococcus Pneumococcal Polysaccharide Type 70 is coated at 2 µg/mL (100 μL/well), Recombinant Human Intelectin-1/Omentin binds with a typical ED50 of 3-15 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Trp17-Arg313
  • Accession #
  • N-terminal Sequence
    Analysis
    Trp17 & Thr19
  • Structure / Form
    Disulfide-linked homotrimer
  • Predicted Molecular Mass
    33 kDa
  • SDS-PAGE
    36-42 kDa, reducing conditions
9137-IN
 
Formulation Lyophilized from a 0.2 μm filtered solution in Tris.
Reconstitution Reconstitute at 500 μg/mL in Water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Streptococcus Pneumococcal Polysaccharide Type 70 is coated at 2 µg/mL (100 μL/well), Recombinant Human Intelectin-1/Omentin binds (Catalog # 9137-IN) with a typical ED50 of
3-15 μg/mL.
Background: Intelectin-1/Omentin
Intelectin-1, also known as Omentin, HL-1, and Lactoferrin Receptor, is a multifunctional protein that plays a protective role in the response to cardiovascular injury (1). Mature human Intelectin-1 contains one fibrinogen C-terminal domain (2-4) and shares 82% and 80% aa sequence identity with mouse and rat Intelectin-1, respectively. Intelectin-1 is expressed by vascular smooth muscle and endothelial cells (3, 5, 6), particularly in the heart, small intestine, colon, thymus, ovary, testis, and omental fat (2, 3, 6) as well as by intestinal Paneth cells and airway and intestinal goblet cells (7-9). It is presented as a 35-40 kDa GPI-anchored glycoprotein that forms disulfide-linked homotrimers (2, 4) and can be released into the circulation and bronchiolar lavage fluid (6, 8). It binds to microbial galactofuranosyl carbohydrates (2, 11) and additionally functions as a receptor for the iron-transporting protein Lactoferrin (4). Following cardiovascular injury, Intelectin-1 limits fibrosis, cardiac hypertrophy, and carotid artery intimal hyperplasia (12, 13) foam cell generation [5], smooth muscle cell proliferation, migration, and calcification (5, 12, 14), and it also promotes revascularization (15). In addition, Intelectin-1 inhibits osteoblast bone matrix mineralization (14), enhances
insulin-stimulated uptake by adipocytes (6), supports neural stem cell viability (16), and inhibits the invasion and metastasis of neuroblastoma cells (17).
  • References:
    1. Jaikanth, C. et al. (2013) Exp. Clin. Endocrinol. Diabetes 121:377.
    2. Tsuji, S. et al. (2001) J. Biol. Chem. 276:23456.
    3. Lee, J.-K. et al. (2001) Glycobiology 11:65.
    4. Suzuki, Y.A. et al. (2001) Biochemistry 40:15771.
    5. Watanabe, K. et al. (2016) Cardiovasc. Res. PMID 26790473.
    6. Yang, R.-Z. et al. (2006) Am. J. Physiol. Endocrinol. Metab. 290:E1253.
    7. Komiya, T. et al. (1998) Biochem. Biophys. Res. Commun. 251:759.
    8. Kerr, S.C. et al. (2014) Am. J. Respir. Crit. Care Med. 189:1005.
    9. Washimi, K. et al. (2012) PLoS One 7:e39889.
    10. Tsuji, S. et al. (2007) Glycobiology 17:1045.
    11. Wesener, D.A. et al. (2015) Nat. Struct. Mol. Biol. 22:603.
    12. Matsuo, K. et al. (2015) J. Mol. Cell. Cardiol. 79:195.
    13. Kazama, K. et al. (2014) Am. J. Physiol. Heart Circ. Physiol. 306:H1714.
    14. Xie, H. et al. (2011) Cardiovasc. Res. 92:296.
    15. Maruyama, S. et al. (2012) J. Biol. Chem. 287:408.
    16. Zhao, L.R. et al. (2015) Mol. Med. Rep. 11:1859.
    17. Li, D. et al. (2015) Mol. Cancer 14:47.
  • Entrez Gene IDs:
    55600 (Human); 16429 (Mouse)
  • Alternate Names:
    Endothelial lectin HL-1; FLJ20022; Galactofuranose-binding lectin; hIntL; HL-1; intelectin 1 (galactofuranose binding); Intelectin1; Intelectin-1; INTLHL1; ITLN1; Itlna; ITLNITLN-1; LFR; LFRIntestinal lactoferrin receptor; Omentin; Omentin-1
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