Recombinant Human PSG-6 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Gln35-His424, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln35 inferred from enzymatic pyroglutamate treatment revealing Val36
  • Predicted Molecular Mass
    45 kDa
  • SDS-PAGE
    62-73 kDa, reducing conditions
9318-P6
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Binding Activity
Recombinant Human PSG-6 (Catalog # 9318-P6) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL.
Background: PSG-6
Pregnancy-specific glycoprotein 6 (PSG-6), also known as PSG10 and PSGGB is a secreted glycoprotein of the PSG family within the CEA (carcinoembryonic antigen) superfamily (1). Mature human PSG-6 is a 401 amino acid (aa) protein that consists of four tandem immunoglobulin-like domains followed by a 30 aa
C-terminal tail (2, 3). The N-terminal Ig-like domain contains an Arg-Gly-Asp (RGD) sequence which is a consensus integrin-binding motif found in many adhesion proteins (4, 5). Alternative splicing generates an additional isoform with a substitution in the C-terminal tail. PSG-6 is predominantly expressed in placenta and more weakly in granulocytes (3). It induces monocyte production of the cytokines IL-6, IL-10, and TGF-beta 1 (6).
  • References:
    1. Kuespert, K. et al. (2006) Curr. Opin. Cell Biol. 18:565.
    2. Zimmermann, W. et al. (1989) Biochem. Biophys. Res. Commun. 163:1197.
    3. Barnett, T.R. et al. (1990) Biochemistry 29:10213.
    4. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
    5. Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
    6. Snyder, S.K. et al. (2001) Am. J. Reprod. Immunol. 45:205.
  • Long Name:
    Pregnancy-specific beta-1-glycoprotein 6
  • Entrez Gene IDs:
    5675 (Human)
  • Alternate Names:
    CGM3; pregnancy specific beta-1-glycoprotein 6; Pregnancy-specific beta-1-glycoprotein 10; Pregnancy-specific beta-1-glycoprotein 12; pregnancy-specific beta-1-glycoprotein 6; Pregnancy-specific glycoprotein 10; Pregnancy-specific glycoprotein 12; Pregnancy-specific glycoprotein 6; PS-beta-G-10; PS-beta-G-12; PS-beta-G-6; PSBG-10; PSBG-12; PSBG-6; PSG10; PSG12; PSG6; PSG-6; PSGGB
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Biotinylated Antibody

WB BAM050 3  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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