Recombinant Mouse CD300c/LMIR8/CLM-6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10183-LM-050
Recombinant Mouse CD300c/LMIR8/CLM-6 Fc Chimera Protein, CF Bioactivity
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Recombinant Mouse CD300c/LMIR8/CLM-6 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.8-4.8 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse CD300c/LMIR8/CLM-6 protein
Mouse CD300c/LMIR8/CLM-6
(His22-Pro187)
Accession # A2A7V7
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
His22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa
SDS-PAGE
55-70 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10183-LM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity <p><br></p> View Larger

Recombinant Mouse CD300c/LMIR8/CLM-6 Fc Chimera (Catalog # 10183-LM) inhibits anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.8-4.8 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse CD300c/LMIR8/CLM-6 Fc Chimera (Catalog # 10183-LM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-70 kDa and 110-140 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300c/LMIR8/CLM-6

CMRF-35-like molecule-6 (CLM-6), known as LMIR8, CMRF-35A1, and CD300c, is a type I transmembrane glycoprotein belonging to the immunoregulatory signaling (IRS) family of immunoglobulin-like molecules (1). There have been 7 human and 9 mouse CD300/CLM family members identified, and they modulate a broad and diverse array of immune cell processes via their paired activating and inhibitory receptor functions (2, 3). Mouse CD300c consists of an extracellular domain (ECD) with one Ig-like domain, a single transmembrane segment, and a short cytoplasmic tail (2). The mature ECD of mouse CD300c is 167 amino acids and shares 48% sequence identity with human CD300c. A charged amino acid residue in the transmembrane domain of CD300c/CLM-6, found in additional family members CLM‑2, CLM-4, CLM-5, and CLM-7, enables interactions with adapter proteins (3,4). CD300c has been shown to be a FcR gamma -coupled receptor that is selectively expressed in Plasmacytoid dendritic cells (pDCs) (5). CD300c signals might negatively regulate the activation of pDCs in response to viral infections (5). Additional research into CD300 receptor function during viral infections could help develop novel anti-viral therapies (6).

References
  1. Clark, G.J. et al. (2001) Tissue Antigens 57:415.
  2. Borrego F. (2013) Blood 121:1951.
  3. Vitallé J. et al. (2019) Eur J Immunol. 49(3):364.
  4. Clark, G.J. et al. (2009) Trends in Immunology 30:209.
  5. Kaitani, A. et al. (2018) Scientific Reports 8:8259.
  6. Graziano, V et al. 2019. Viruses. 11(6):498.
Long Name
CD300C Molecule 2
Entrez Gene IDs
10871 (Human); 387565 (Mouse)
Alternate Names
CD300c/LMIR8/CLM-6; CLM6; CLM-6; CMRF35; IGSF16

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