Recombinant Mouse CD300c/LMIR8/CLM-6 Fc Chimera Protein, CF Summary
Accession # A2A7V7
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse CD300c/LMIR8/CLM-6 Fc Chimera (Catalog # 10183-LM) inhibits anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.8-4.8 μg/mL.
2 μg/lane of Recombinant Mouse CD300c/LMIR8/CLM-6 Fc Chimera (Catalog # 10183-LM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-70 kDa and 110-140 kDa, respectively.
CMRF-35-like molecule-6 (CLM-6), known as LMIR8, CMRF-35A1, and CD300c, is a type I transmembrane glycoprotein belonging to the immunoregulatory signaling (IRS) family of immunoglobulin-like molecules (1). There have been 7 human and 9 mouse CD300/CLM family members identified, and they modulate a broad and diverse array of immune cell processes via their paired activating and inhibitory receptor functions (2, 3). Mouse CD300c consists of an extracellular domain (ECD) with one Ig-like domain, a single transmembrane segment, and a short cytoplasmic tail (2). The mature ECD of mouse CD300c is 167 amino acids and shares 48% sequence identity with human CD300c. A charged amino acid residue in the transmembrane domain of CD300c/CLM-6, found in additional family members CLM‑2, CLM-4, CLM-5, and CLM-7, enables interactions with adapter proteins (3,4). CD300c has been shown to be a FcR gamma -coupled receptor that is selectively expressed in Plasmacytoid dendritic cells (pDCs) (5). CD300c signals might negatively regulate the activation of pDCs in response to viral infections (5). Additional research into CD300 receptor function during viral infections could help develop novel anti-viral therapies (6).
- Clark, G.J. et al. (2001) Tissue Antigens 57:415.
- Borrego F. (2013) Blood 121:1951.
- Vitallé J. et al. (2019) Eur J Immunol. 49(3):364.
- Clark, G.J. et al. (2009) Trends in Immunology 30:209.
- Kaitani, A. et al. (2018) Scientific Reports 8:8259.
- Graziano, V et al. 2019. Viruses. 11(6):498.
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