Immobilized Recombinant Mouse CD8 alpha (Catalog # 9135-CD) supports the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 1-5 μg/mL.
CD8, also known as Ly-2 or Leu-2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4+CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma-delta T cells (7), subsets of thymic and splenic dendritic cells (DC) (8), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8+ DC express primarily alpha beta heterodimers, while splenic CD8+ DC primarily express alpha alpha homodimers (8). CD8 alpha + DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively.
Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
Cole, D.K. et al. (2012) Immunology 137:139.
Germain, R.N. (2002) Nat. Rev. Immunol. 2:309.
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
Nakayama, K. et al. (1994) Science 263:1131.
Wang, J. and J.R. Klein (1994) Science 265:1860.
MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
Vremec, D. et al. (1992) J. Exp. Med. 176:47.
Lin, T.-J. et al. (1998) J. Immunol. 161:6265.
Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
Belz, G.T. et al. (2004) J. Immunol. 172:1996.
Nakauchi, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5126.
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