Recombinant Mouse IGF-I Protein, CF

  (42 citations)     
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Citations (42)
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  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.4-2 ng/mL.
  • Source
    E. coli-derived mouse IGF-I protein
    Gly33-Ala102
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly33
  • Predicted Molecular Mass
    7.6 kDa
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791-MG
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Images
Recombinant Mouse IGF-I (Catalog #
791-MG) stimulates cell proliferation in a serum-free assay using the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.4-2 ng/mL.
1 µg/lane of Recombinant Mouse IGF-I was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.
Background: IGF-I

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse
IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4 - 7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds
IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

  • References:
    1. Philippou, A. et al. (2007) In Vivo 21:45.
    2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
    3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
    4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
    5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
    6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
    7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
    8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
    9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
    10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
  • Long Name:
    Insulin-like Growth Factor I
  • Entrez Gene IDs:
    3479 (Human); 16000 (Mouse); 24482 (Rat)
  • Alternate Names:
    IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Impact of insulin on primary arcuate neurons culture is dependent on early-postnatal nutritional status and neuronal subpopulation
    Authors: L Decourtye, M Clemessy, E Mire, T Ledent, L Périn, IC Robinson, Y Le Bouc, L Kappeler
    PLoS ONE, 2018;13(2):e0193196.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  2. Expression of antisense of microRNA-26a-5p in mesenchymal stem cells increases their therapeutic effects against cirrhosis
    Authors: L Chen, W Zeng, B Yang, X Cui, C Feng, L Wang, H Wang, X Zhou, P Li, F Lv, T Li
    Am J Transl Res, 2017;9(3):1500-1508.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. The type I insulin-like growth factor regulates the liver stromal response to metastatic colon carcinoma cells
    Authors: MC Fernandez, R Rayes, B Ham, N Wang, F Bourdeau, S Milette, M Lllemann, N Bird, A Majeed, J Xu, T Kisselova, P Brodt
    Oncotarget, 2017;8(32):52281-52293.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin
    Authors: NJ Huang, N Pishesha, J Mukherjee, S Zhang, R Deshycka, V Sudaryo, M Dong, CB Shoemaker, HF Lodish
    Nat Commun, 2017;8(1):423.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Microarray analyses of otospheres derived from the cochlea in the inner ear identify putative transcription factors that regulate the characteristics of otospheres
    Authors: T Iki, M Tanaka, SI Kitajiri, T Kita, Y Kawasaki, A Mizukoshi, W Fujibuchi, T Nakagawa, T Nakahata, J Ito, K Omori, MK Saito
    PLoS ONE, 2017;12(6):e0179901.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  6. IGFBP7 Deletion Promotes Hepatocellular Carcinoma
    Authors: M Akiel, C Guo, X Li, D Rajasekara, RG Mendoza, CL Robertson, N Jariwala, F Yuan, MA Subler, J Windle, DK Garcia, Z Lai, HH Chen, Y Chen, S Giashuddin, PB Fisher, XY Wang, D Sarkar
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Insulin-like growth factor 1 receptor regulates hypothermia during calorie restriction
    Authors: R Cintron-Co, M Sanchez-Al, W Nguyen, S Mori, R Gonzalez-R, T Lien, T Bartfai, S Aïd, JC François, M Holzenberg, B Conti
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  8. Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
    Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Protein Kinase C Epsilon Cooperates with PTEN Loss for Prostate Tumorigenesis through the CXCL13-CXCR5 Pathway
    Authors: R Garg, JM Blando, CJ Perez, MC Abba, F Benavides, MG Kazanietz
    Cell Rep, 2017;19(2):375-388.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. Cancer stem cells regulate cancer-associated fibroblasts via activation of Hedgehog signaling in mammary gland tumors
    Authors: G Valenti, HM Quinn, GJ Heynen, L Lan, JD Holland, R Vogel, A Wulf-Golde, W Birchmeier
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. IGF-1 degradation by mouse mast cell protease 4 promotes cell death and adverse cardiac remodeling days after a myocardial infarction
    Authors: Thor Tejada
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: Protein
    Application: Bioassay
  12. Exercise restores muscle stem cell mobilization, regenerative capacity and muscle metabolic alterations via adiponectin/AdipoR1 activation in SAMP10 mice
    Authors: Aiko Inoue
    J Cachexia Sarcopenia Muscle, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
  13. Smad2/3 Proteins Are Required for Immobilization-induced Skeletal Muscle Atrophy
    Authors: T Tando, A Hirayama, M Furukawa, Y Sato, T Kobayashi, A Funayama, A Kanaji, W Hao, R Watanabe, M Morita, T Oike, K Miyamoto, T Soga, M Nomura, A Yoshimura, M Tomita, M Matsumoto, M Nakamura, Y Toyama, T Miyamoto
    J. Biol. Chem., 2016;291(23):12184-94.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Bioassay
  14. EZH1 and EZH2 promote skeletal growth by repressing inhibitors of chondrocyte proliferation and hypertrophy
    Nat Commun, 2016;7(0):13685.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. Bmi1 Loss in the Organ of Corti Results in p16ink4a Upregulation and Reduced Cell Proliferation of Otic Progenitors In Vitro
    PLoS ONE, 2016;11(10):e0164579.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination with Cell Polarization During Ameloblast Differentiation
    J Bone Miner Res, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  17. Mycobacterium leprae-induced Insulin-like Growth Factor I attenuates antimicrobial mechanisms, promoting bacterial survival in macrophages
    Authors: L R Batista-Si
    Sci Rep, 2016;6(0):27632.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Proliferation of cultured mouse choroid plexus epithelial cells.
    Authors: Barkho B, Monuki E
    PLoS ONE, 2015;10(3):e0121738.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions.
    Authors: Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S
    PLoS ONE, 2015;10(1):e0117908.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. Intra-myocardial injection of both growth factors and heart derived Sca-1+/CD31- cells attenuates post-MI LV remodeling more than does cell transplantation alone: neither intervention enhances functionally significant cardiomyocyte regeneration.
    Authors: Wang X, Li Q, Hu Q, Suntharalingam P, From A, Zhang J
    PLoS ONE, 2014;9(2):e95247.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. HGF signaling impacts severity of Pseudomonas aeruginosa keratitis.
    Authors: Jiang X, McClellan S, Barrett R, Foldenauer M, Hazlett L
    Invest Ophthalmol Vis Sci, 2014;55(4):2180-90.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  22. BMP4 is required for the initial expression of MITF in melanocyte precursor differentiation from embryonic stem cells.
    Authors: Yang J, Wang J, Pan L, Li H, Rao C, Zhang X, Niu G, Qu J, Hou L
    Exp Cell Res, 2014;320(1):54-61.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. Myostatin stimulates, not inihibits, C2C12 myoblast proliferation.
    Authors: Rodgers B, Wiedeback B, Hoversten K, Jackson M, Walker R, Thompson T
    Endocrinology, 2014;155(3):670-5.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  24. Factors expressed by murine embryonic pancreatic mesenchyme enhance generation of insulin-producing cells from hESCs.
    Authors: Guo T, Landsman L, Li N, Hebrok M
    Diabetes, 2014;62(5):1581-92.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  25. Comparing the cultivated cochlear cells derived from neonatal and adult mouse.
    Authors: Lou, Xiangxin, Dong, Youyi, Xie, Jing, Wang, Xianliu, Yang, Lianglia, Tokuda, Masaaki, Zhang, Yanzhong
    J Transl Med, 2014;12(0):150.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  26. A novel molecule Me6TREN promotes angiogenesis via enhancing endothelial progenitor cell mobilization and recruitment.
    Authors: Chen H, Wang S, Zhang J, Ren X, Zhang R, Shi W, Lv Y, Zhou Y, Yan X, Chen L, He L, Zhang B, Nan X, Yue W, Li Y, Pei X
    Sci Rep, 2014;4(0):6222.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  27. G(i)alpha proteins exhibit functional differences in the activation of ERK1/2, Akt and mTORC1 by growth factors in normal and breast cancer cells.
    Authors: Wang Z, Dela Cruz R, Ji F, Guo S, Zhang J, Wang Y, Feng G, Birnbaumer L, Jiang M, Chu W
    Cell Commun Signal, 2014;12(0):10.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. Calorie restriction decreases murine and human pancreatic tumor cell growth, nuclear factor-kappaB activation, and inflammation-related gene expression in an insulin-like growth factor-1-dependent manner.
    Authors: Harvey A, Lashinger L, Hays D, Harrison L, Lewis K, Fischer S, Hursting S
    PLoS ONE, 2014;9(5):e94151.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  29. The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function.
    Authors: Takehara, Yuji, Yabuuchi, Akiko, Ezoe, Kenji, Kuroda, Tomoko, Yamadera, Rie, Sano, Chiaki, Murata, Nana, Aida, Takuya, Nakama, Ken, Aono, Fumihito, Aoyama, Naoki, Kato, Keiich, Kato, Osamu
    Lab Invest, 2013;93(2):181-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: Bioassay
  30. IGF1 receptor signaling regulates adaptive radioprotection in glioma stem cells.
    Authors: Osuka S, Sampetrean O, Shimizu T, Saga I, Onishi N, Sugihara E, Okubo J, Fujita S, Takano S, Matsumura A, Saya H
    Stem Cells, 2013;31(4):627-40.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  31. Exercise induces hippocampal BDNF through a PGC-1alpha/FNDC5 pathway.
    Authors: Wrann C, White J, Salogiannnis J, Laznik-Bogoslavski D, Wu J, Ma D, Lin J, Greenberg M, Spiegelman B
    Cell Metab, 2013;18(5):649-59.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Cell Culture Substrate
  32. Recombinant insulin-like growth factor-1 activates satellite cells in the mouse urethral rhabdosphincter.
    Authors: Wei W, Howard P, Macarak E
    BMC Urol, 2013;13(0):62.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  33. Age-Dependent Neuroimmune Modulation of IGF-1R in the Traumatic Mice.
    Authors: Zhao H, Zhao X, Cao X, Wu G
    Immun Ageing, 2012;9(1):12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  34. IGFBP-3 is a metastasis suppression gene in prostate cancer.
    Authors: Mehta HH, Gao Q, Galet C
    Cancer Res., 2011;71(15):5154-63.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  35. Insulin-like Growth Factor 1-mediated Hyperthermia Involves Anterior Hypothalamic Insulin Receptors.
    Authors: Sanchez-Alavez M, Osborn O, Tabarean IV
    J. Biol. Chem., 2011;286(17):14983-90.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  36. A critical role for IGF-II in memory consolidation and enhancement.
    Authors: Chen DY, Stern SA, Garcia-Osta A, Saunier-Rebori B, Pollonini G, Bambah-Mukku D, Blitzer RD, Alberini CM
    Nature, 2011;469(7331):491-7.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  37. Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation.
    Authors: Yamada PM, Mehta HH, Hwang D
    Endocrinology, 2010;151(12):5741-50.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  38. The chaperone activity of GRP94 toward insulin-like growth factor II is necessary for the stress response to serum deprivation.
    Authors: Ostrovsky O, Ahmed NT, Argon Y
    Mol. Biol. Cell, 2009;20(6):1855-64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  39. Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma.
    Authors: Hu J, Dong A, Fernandez-Ruiz V, Shan J, Kawa M, Martinez-Anso E, Prieto J, Qian C
    Cancer Res., 2009;69(17):6951-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  40. Insulin-like growth factor-1 improves survival in sepsis via enhanced hepatic bacterial clearance.
    Authors: Ashare A, Nymon AB, Doerschug KC, Morrison JM, Monick MM, Hunninghake GW
    Am. J. Respir. Crit. Care Med., 2008;178(2):149-57.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  41. Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model.
    Authors: Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R
    Cancer Res., 2007;67(22):11083-91.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  42. Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
    Authors: Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M
    Mol. Cell. Neurosci., 2006;31(1):149-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
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