Recombinant Viral HCV E2, CF

Hepatitis C Virus 1b Envelope Glycoprotein E2
  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is coated onto a microplate at 2 μg/mL, Recombinant Viral HCV E2 binds with an ED50 of 0.2-1.2 µg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Glu384-Glu661, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Glu384
  • Predicted Molecular Mass
    31 kDa
  • SDS-PAGE
    63-74 kDa, reducing conditions
9146-HC
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is coated onto a microplate at 2 μg/ml, Recombinant Viral HCV E2 (Catalog # 9146-HC) bindswith an ED50 of 0.2-1.2 µg/mL.
Background: HCV E2
Glycoprotein E2 is an approximately 60 kDa viral envelope protein encoded by the hepatitis C virus (HCV) genome (1-3). HCV infection is closely associated with the development of hepatocellular carcinoma and cirrhosis of the liver. E2 is a transmembrane molecule expressed as a noncovalent dimer with the E1 glycoprotein (4). It binds to CD81 and CD36/SR-BI on hepatocytes, and glycosylation of E2 is important for this interaction (4-8). The virus also requires target cell expression of the tight junction proteins Claudin-1 and Occludin for infectivity (7, 9, 10). CD81 and Occludin are responsible for the human host cell specificity of HCV (10). E2 binding to CD81 on NK cells inhibits cytokine production and cytolytic activity (11, 12). In contrast, E2 binding to CD81 on T cells provides a costimulatory signal (11, 12). On plasmacytoid dendritic cells, E2 binds to CD81 as well as BDCA-2 and DCIR, resulting in inhibition of IFN-alpha production and cell maturation (13, 14).
  • References:
    1. Khan, A.G. et al. (2015) Curr. Opin. Virol. 12:53.
    2. Zona, L. et al. (2014) Viruses 6:875.
    3. Grakoui, A. et al. (1993) J. Virol. 67:1385.
    4. Op De Beeck, A. et al. (2004) J. Virol. 78:2994.
    5. Pileri, P. et al. (1998) Science 282:938.
    6. Scarselli, E. et al. (2002) EMBO J. 21:5017.
    7. Harris, H.J. et al. (2010) J. Biol. Chem. 285:21092.
    8. Falkowska, E. et al. (2007) J. Virol. 81:8072.
    9. Evans, M.J. et al. (2007) Nature 446:801.
    10. Ploss, A. et al. (2009) Nature 457:882.
    11. Crotta, S. et al. (2002) J. Exp. Med. 195:35.
    12. Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
    13. Tu, Z. et al. (2013) Cell. Immunol. 284:98.
    14. Florentin, J. et al. (2012) Blood 120:4544.
  • Long Name:
    Hepatitis C Virus 1b Envelope Glycoprotein E2
  • Entrez Gene IDs:
    951475 (Viral)
  • Alternate Names:
    HCV E2; HCV-E2; hcvGP-E2; Hep C E2
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 25  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Biotinylated Antibody

WB BAM050 3  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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