>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human CD81 LEL Fc Chimera
is coated onto a microplate at 2 μg/mL, Recombinant Viral HCV E2 binds with an ED50 of 0.2-1.2 µg/mL.
Human embryonic kidney cell, HEK293-derived Glu384-Glu661, with a C-terminal 6-His tag
When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is coated onto a microplate at 2 μg/ml, Recombinant Viral HCV E2 (Catalog # 9146-HC) bindswith an ED50 of 0.2-1.2 µg/mL.
Background: HCV E2
Glycoprotein E2 is an approximately 60 kDa viral envelope protein encoded by the hepatitis C virus (HCV) genome (1-3). HCV infection is closely associated with the development of hepatocellular carcinoma and cirrhosis of the liver. E2 is a transmembrane molecule expressed as a noncovalent dimer with the E1 glycoprotein (4). It binds to CD81 and CD36/SR-BI on hepatocytes, and glycosylation of E2 is important for this interaction (4-8). The virus also requires target cell expression of the tight junction proteins Claudin-1 and Occludin for infectivity (7, 9, 10). CD81 and Occludin are responsible for the human host cell specificity of HCV (10). E2 binding to CD81 on NK cells inhibits cytokine production and cytolytic activity (11, 12). In contrast, E2 binding to CD81 on T cells provides a costimulatory signal (11, 12). On plasmacytoid dendritic cells, E2 binds to CD81 as well as BDCA-2 and DCIR, resulting in inhibition of IFN-alpha production and cell maturation (13, 14).
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