rhOGG1 FLARE Assay Kit
rhOGG1 FLARE Assay Kit Summary
The hOGG1 FLARE (Fragment Length Analysis using Repair Enzymes) Assay Kit provides the ability to probe for oxidative DNA damage in single cells using hOGG1 in conjunction with comet assay. The kit includes the hOGG1 enzyme and buffers, comet assay reagents, and FLARE slides.
• FLARE Slides (3 Well, 25 Slides)
• CometAssay Lysis Solution
• CometAssay LMAgarose
• REC™ Dilution Buffer
• 25X FLARE Buffer 1
• 100X BSA Additive
• E. coli hOGG1
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
For research use only. Not for diagnositic use.
Citations for rhOGG1 FLARE Assay Kit
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Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation
Authors: H Kurita, N Haruta, Y Uchihashi, T Seto, K Takashima
PLoS ONE, 2020;15(5):e0232724. 2020
Evidence for different mechanisms of action behind the mutagenic effects of 4-NOPD and OPD: the role of DNA damage, oxidative stress and an imbalanced nucleotide pool.
Authors: Asgard R, Haghdoost S, Golkar S, Hellman B, Czene S
Mutagenesis, 0;28(6):637-44. 0
What type of coverslips are used to distribute enzyme over the wells in the FLARE™ Assay?
After adding Enzyme Solution, wells can be covered with a hydrophobic coverslip (Catalog # 4867-100) to evenly distribute the enzyme test dilution.
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