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rhOGG1 FLARE Assay Kit

4130-100-FK 2 Citations Datasheet / COA / SDS
This product is in the process of being discontinued

Discontinued Product

4130-100-FK has been discontinued.
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Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Background

rhOGG1 FLARE Assay Kit Summary

Provides the ability to probe for oxidative DNA damage in single cells using hOGG1 in conjunction with comet assay.

Product Description

The hOGG1 FLARE (Fragment Length Analysis using Repair Enzymes) Assay Kit provides the ability to probe for oxidative DNA damage in single cells using hOGG1 in conjunction with comet assay. The kit includes the hOGG1 enzyme and buffers, comet assay reagents, and FLARE slides.

Kit Contents

• FLARE Slides (3 Well, 25 Slides)
• CometAssay Lysis Solution
• CometAssay LMAgarose
• REC™ Dilution Buffer
• 25X FLARE Buffer 1
• 100X BSA Additive
• E. coli hOGG1

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Human

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Product Datasheet
COA
SDS

Citations for rhOGG1 FLARE Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation
    Authors: H Kurita, N Haruta, Y Uchihashi, T Seto, K Takashima
    PLoS ONE, 2020;15(5):e0232724.  2020
  2. Evidence for different mechanisms of action behind the mutagenic effects of 4-NOPD and OPD: the role of DNA damage, oxidative stress and an imbalanced nucleotide pool.
    Authors: Asgard R, Haghdoost S, Golkar S, Hellman B, Czene S
    Mutagenesis, 0;28(6):637-44.  0

FAQs

  1. What type of coverslips are used to distribute enzyme over the wells in the FLARE™ Assay?

    • After adding Enzyme Solution, wells can be covered with a hydrophobic coverslip (Catalog # 4867-100) to evenly distribute the enzyme test dilution.

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