SARS-CoV-2 NSP7 Antibody

Catalog # Availability Size / Price Qty
SARS-CoV-2 NSP7 ELISA Standard Curve.
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Product Details
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SARS-CoV-2 NSP7 Antibody Summary

Species Reactivity
Detects SARS-CoV-2 NSP7 in ELISA.
Monoclonal Mouse IgG1 Clone # 1045516
Protein A or G purified from cell culture supernatant
E. coli-derived SARS-CoV-2 NSP7 protein
Accession # YP_009725303.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Scientific Data

SARS-CoV-2 NSP7 ELISA Standard Curve. Recombinant SARS-CoV-2 NSP7 protein was serially diluted 2-fold and captured by Mouse Anti-SARS-CoV-2 NSP7 Monoclonal Antibody (Catalog # MAB10990) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-SARS-CoV-2 NSP7 Monoclonal Antibody (Catalog # MAB10994) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NSP7

Non-structural protein 7 (NSP7) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV-2 that have had significant impact on humans in addition to strains that have negatively impacted livestock. NSP7 is a small 83 amino acid protein that is highly conserved across coronaviruses (1). The NSP7 monomers are composed of a central core N-terminal tri-helical bundle with an additional short C-terminal helix (2). The monomeric units associate to form a large hexadecameric structure with NSP8 (2) where layers of NSP7 fill the spaces in between NSP8 units. The supercomplexes are stacked to form a channel with electrostatic properties that could allow RNA to pass through the channel to facilitate efficient replication and transcription. The NSP7/NSP8 supercomplex was thereby proposed to function as a primase for the viral RNA-dependent RNA polymerase (RdRp), NSP12 (3). In SARS-CoV-2, the RdRp has been shown to have little activity without NSP8/7 as cofactors (4) making NSP7/NSP8 critical for viral polymerase activity. NSP7 has also been shown to interact with several other viral NSP proteins, including NSP5, NSP9, and NSP13 (5) as well as multiple host cell proteins involved in membrane trafficking, signaling, and electron transport including potential drug targets such as COMT and PTGES (6). 

  1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59.
  2. Zhai, Y. et al. (2005) Nat. Struct. Mol. Bio. 12:980.
  3. Subissi, L. et al. (2014) Antiviral Res. 101:122.
  4. Yin, W. et al. (2020) Science 368:1499.
  5. von Brunn, A. et al. (2007) PLoS One 2:e459.
  6. Gordon, D.E. et al. (2020) Nature 583:459.
Long Name
Non-Structural Protein 7
Alternate Names

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