Detects heavy ( gamma ) chains on sheep IgG and light chains on all sheep immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin sheep serum proteins or IgG from human, mouse or rabbit, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Polyclonal Donkey IgG
Supplied as a solution in PBS with preservative.
Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Progesterone R/NR3C3 in Human Breast Cancer Tissue. Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) with Anti-Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). View our protocol for the VisUCyte HRP Polymer Detection Reagent.
Preparation and Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
6 months from date of receipt, 2 to 8 °C as supplied.
VisUCyte™ HRP Polymer is a
biotin-free detection reagent which overcomes problems of avidin-biotin
detection chemistry, such as endogenous biotin staining and the need to do
additional quenching of endogenous biotin and avidin present in
some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells
and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may
be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.