hh Sheep IgG PerCP-conjugated Antibody: R&D Systems

Sheep IgG PerCP-conjugated Antibody

IC016C has been discontinued. View all IgG products.
  
  • Specificity
    Serum was obtained from naive (non-immunized) sheep.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Protein A or G purified
  • Immunogen
    NA
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Sheep IgGControl by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) werestained with Sheep Anti-Human IRF5 PerCP-conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog # IC4508C,filled histogram) or SheepIgG PerCP-conjugated Isotype Control Antibody (Catalog # IC016C, openhistogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #FC004)and permeabilized with Flow Cytometry Permeabilization/WashBuffer I (Catalog #FC005).View our protocol for StainingIntracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

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