|Detection of Sheep IgGControl by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) werestained with Sheep Anti-Human IRF5 PerCP-conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog # IC4508C,filled histogram) or SheepIgG PerCP-conjugated Isotype Control Antibody (Catalog # IC016C, openhistogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #FC004)and permeabilized with Flow Cytometry Permeabilization/WashBuffer I (Catalog #FC005).View our protocol for StainingIntracellular Molecules.|
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
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