Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of Sheep IgGControl by Flow Cytometry
Human peripheral blood mononuclear cells (PBMCs) werestained with Sheep Anti-Human IRF5 PerCP-conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog # IC4508C,filled histogram) or SheepIgG PerCP-conjugated Isotype Control Antibody (Catalog # IC016C, openhistogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #FC004)and permeabilized with Flow Cytometry Permeabilization/WashBuffer I (Catalog #FC005).View our protocol for StainingIntracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
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