Simple Plex Human MMP-1 Cartridge

Simple Plex Cartridge Kit for 72 samples, containing MMP-1 for use with Human Plasma/Serum. Includes wash buffer and sample diluent SD06. For use with the Ella automated immunoassay system

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Human MMP-1 Simple Plex Assay
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Simple Plex Human MMP-1 Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
5.7 pg/mL
Assay Range
5.7 - 11,800 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human MMP-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human MMP-1 in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assa
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean 71.4 3593 71.6 3583
Standard Deviation 4.91 215 3.03 141
CV% 6.9 6 4.2 3.9

Recovery

Recovery was not necessary as MMP-1 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human MMP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human MMP-1 Simple Plex Assay Linearity

Data Examples

Human MMP-1 Simple Plex Assay

Human MMP-1 Simple Plex Assay Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-1

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 1
Entrez Gene IDs:
4312 (Human); 83995 (Mouse)
Alternate Names:
CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 3.4.24.7; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP1; MMP-1

FAQs

No product specific FAQs exist for this product, however you may

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