TcBuster™ Gene Transfer System

Cell Engineering

Your Favorite Genes... In Your Favorite Cell

TcBuster is a next-generation non-viral genetic modification system with the potential to deliver therapeutic payloads to various cell types to address a broad range of therapeutic applications.

Along with other non-viral gene therapy platforms, TcBuster has been extensively studied as a tool for intracellular gene transfer for cell and gene therapy. TcBuster can revolutionize your biological research and can provide you with the tool you need to make useful cell lines, produce proteins, vaccines and much more.

TcBuster addresses your challenges with:

  • the high cost of virus-based genetic modification
  • the inaccessibility of virus-based modification reagents
  • the cargo size constraints of virus-based systems

Let us help you create novel cell lines to address your unique applications.

 

Solve Your Gene Transfer Problems with TcBuster

 

Non-viral Gene Delivery Using DNA Transposons

Non-viral Gene Delivery Using DNA Transposons for Clinical Application

 

 

TcBuster - Non-Viral Transgene Insertion

TcBuster is a core technology in our methods and research. Transposons have demonstrated the safest insertion profile of any gene delivery system while maintaining high efficiency gene transfer and enable us to rapidly generate transgenic mammalian cells with limited off-target effects and stable, reliable gene expression.

 

 

Advantages of Non-Viral Delivery

Our high efficiency non-viral TcBuster system enables therapeutic companies to reduce costs, ensure supply chain stability, and produce safer products.

Non-viral delivery in contrast to lentivirus gene delivery:

  • lower cost per patient.
  • does not provoke an immune response.
  • does not carry mutagenic risks.
  • shows few preferences for integration sites.
  Viral Gene Editing Nonviral Gene Editing
  Lentivirus TcBuster
Cost +++ +
Consistency Lot-to-lot variability
Supply shortages
Scalable
Repeatable
Capacity Limited size of cargo
>Max 9kb
Demonstrated ability to transfer multiple genes
>11kb
Safety Random integration;
Preference for:
- exons
- transcriptional start sites
Random integration;
No preference
Mediates stable gene transfer Yes Yes
Used clinically +++ ++
Immunogenicity Yes No

 

 

Overcome Limits of Electroporation

Our cutting edge non-viral vector transgene insertion platform harnesses DNA transposons for stable gene transfer into any cell type. TcBuster overcomes the three major technical challenges of other transposon systems.

  1. Insertion efficiency - greater than 40% insertion efficiency for stable integration in T cells
  2. DNA toxicity - proprietary techniques reduce effects of DNA toxicity
  3. Integration of enzyme DNA into host genome - delivery of transposase as mRNA or protein.

DNA transposons are a core technology in our methods and research. Transposons have demonstrated the safest insertion profile of any gene delivery system while maintaining high efficiency gene transfer. DNA transposons enable rapid generation of transgenic mammalian cells with limited off-target effects and stable, reliable gene expression.

graphic of TcBuster transposon gene delivery

 

Transposons: How Do We Compare?

  Viral Gene Editing Viral Gene Editing Nonviral Gene Editing
  Sleeping Beauty piggyBac TcBuster
Source Salmonid fossils Cabbage Looper Red Flour Beetle
Provider Ziopharm/Merck Transpoagen/Posieda R&D Systems
Performance In the clinic In the clinic 40% Transposition efficiency
Availability Unavailable (Exclusively Licensed) Unavailable (Exclusively Licensed) Available