Viral B19R Antibody

  
  • Species Reactivity
    Viral
  • Specificity
    Detects viral B19R in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Human embryonic kidney cell line HEK293-derived recombinant viral B19R
    His20-Glu351
    Accession # P25213
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Viral B19R by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with viral B19R. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Viral B19R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for B19R at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B19R
B19R is a 60-65 kDa protein encoded by the Vaccinia virus genome and by the genomes of other orthopoxviruses. Its function represents one of several mechanisms used by these viruses to evade the host immune response. It is known as B19R in the Copenhagen strain of Vaccinia and as B18R in the Western Reserve (WR) strain. There is a structurally-unrelated, larger Vaccinia protein that is also known as B18R that contains multiple ankyrin-like repeats. B19R, however, contains three immunoglobulin-like domains and shows homology to human, mouse, and bovine type I interferon receptors. The Wyeth strain of Vaccinia virus encodes a truncated protein that lacks the C-terminal Ig-like domain, and the Lister strain of Vaccinia produces an inactive B19R. B19R functions as a decoy binding protein for type I interferons (IFN alpha, beta, omega). It binds to type I interferons from multiple species and prevents IFN signaling through its receptors. B19R binds to the surface of virus infected and uninfected cells where it retains its capacity to bind and neutralize IFN. It shields those cells from the antiviral effects of type I interferons, thereby enabling virus replication and pathogenicity. B19R also limits the effectiveness of IFN alpha produced following TLR activation, and it limits adaptive T cell responses.
  • Long Name:
    Soluble Interferon alpha/beta Receptor B19
  • Entrez Gene IDs:
    3707577 (Viral)
  • Alternate Names:
    B19R
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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