Viral HHV8-ORF74 PE-conjugated Antibody

FAB5264P has been discontinued. View all HHV8-ORF74 products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects viral HHV8-ORF74 in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 462510
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with viral HHV8-ORF74
    Accession # AAB51506
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of HHV8‑ORF74 in HEK293 Human Cell Line Transfected with Viral HHV8-ORF74 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with viral HHV8-ORF74 and eGFP was stained with either (A) Mouse Anti-Viral HHV8‑ORF74 PE‑conjugated Monoclonal Antibody (Catalog # FAB5264P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HHV8-ORF74
The herpes family represents about 130-140 double-stranded DNA viruses that infect almost all phyla in the animal kingdom. HHV8 (Human Herpes Virus-8) is one of eight herpes viruses known to infect humans. These eight viruses are categorized into three classes: alpha (HHV1-3 that infect neurons), beta (HHV5-7) and gamma (HHV4 and 8 that lie latent in B cells). HHV8 contains about 100 genes/ORFs, including viral homologues for MIP (vMIP), Bcl-2, IL-6, NCAM and IL-8 R2. HHV8-ORF74 is a homolog of IL-8 R2, and when expressed shows a 44-46 kDa G-protein coupled receptor that belongs to the rhodopsin/ beta -adrenergic family of GCPRs. Unlike IL-8 R2, HHV8-ORF74 is constitutively active. Its activity is regulated by multiple chemokines that can either upregulate basal activity (IL-8; GRO-alpha ), downregulate basal activity (IP-10; vMIP-II; SFD-1) or leave activity untouched (NAP-2; ENA-78), acting instead as a blocking factor. HHV8-ORF74 activation on infected endothelial cells appears to drive them into a spindle-shape, characteristic of that seen in Kaposi's sarcoma, and induces the expression of multiple proangiogenic molecules such as IL-6, IL-8, ICAM-1, VCAM-1 and VEGF plus VEGF R2. These effects appear to be necessary, but not necessarily sufficient, for Kaposi's sarcoma development. The ORF74 GPCR shares 29% and 27% amino acid sequence identity with human CXCR2 and CXCR1, respectively.
  • Long Name:
    Human Herpesvirus 8 ORF74
  • Entrez Gene IDs:
    4961460 (Viral)
  • Alternate Names:
Related Research Areas


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