Viral Tick-borne Encephalitis Virus Envelope Antibody

Catalog # Availability Size / Price Qty
MAB101061-100
MAB101061-SP
Viral Tick-borne Encephalitis Virus Envelope Antibody in Western Blot
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Viral Tick-borne Encephalitis Virus Envelope Antibody Summary

Species Reactivity
Viral
Specificity
Detects Viral Tick-borne Encephalitis Virus Envelope in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1004120
Purification
Protein A or G purified from ascites
Immunogen
Human embryonic kidney cell line HEK293-derived viral Tick-borne Encephalitis Virus Envelope
Amino Acids 777-1128
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Viral Tick-borne Encephalitis Virus Envelope Monoclonal Antibody (Catalog # MAB10106).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Tick-borne Encephalitis Virus Envelope by Western Blot. View Larger

Detection of Tick-borne Encephalitis Virus Envelope by Western Blot. Western blot shows recombinant tick-borne encephalitis virus (TBEV). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Tick-borne Encephalitis Virus Envelope Monoclonal Antibody (Catalog # MAB101061) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tick-borne Encephalitis Virus Envelope at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Viral Tick-borne Encephalitis Virus Envelope ELISA Standard Curve. Recombinant Viral Tick-borne Encephalitis Virus Envelope protein was serially diluted 2-fold and captured by Mouse Anti-Viral Tick-borne Encephalitis Virus Envelope Monoclonal Antibody (Catalog # MAB101061) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Tick-borne Encephalitis Virus Envelope Monoclonal Antibody (Catalog # MAB10106)  was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tick-borne Encephalitis Virus Envelope

Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus, is the virus associated with tick-borne encephalitis. Three TBEV sub-types have been described: European or Western tick-borne encephalitis virus, Siberian tick-borne encephalitis virus, and Far eastern Tick-borne encephalitis virus (formerly known as Russian Spring Summer encephalitis virus, RSSEV). TEBV NS1 protein is one of the most conserved. Homology level of the NS1 protein is about 90% between TBEV strains. Tick-borne encephalitis virus causes 13,000 cases of human meningitis and encephalitis annually.

Entrez Gene IDs
1489719 (Viral)
Alternate Names
TBEV_E; TBEV-E; TBEV-Env; Tick-borne Encephalitis Virus Envelope

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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