Zika Virus NS1 Antibody

Novus Biologicals | Catalog # NBP3-48633

Novus Biologicals
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Key Product Details

Species Reactivity

Viral

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Zika Virus NS1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Zika Virus NS1 (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Zika Virus NS1 Antibody

Zika Virus NS1 Antibody

Western Blot: Zika Virus NS1 Antibody [NBP3-48633] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika Virus NS1 antibody (NBP3-48633) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Zika Virus NS1 Antibody

Immunocytochemistry/ Immunofluorescence: Zika Virus NS1 Antibody [NBP3-48633] -

Zika Virus NS1 antibody detects Zika Virus NS1 at cytoplasm by immunofluorescent analysis.
Sample: Vero cells were fixed in 4% paraformaldehyde at RT for 30 min.
Green: Zika virus NS1 stained by Zika Virus NS1 antibody (NBP3-48633) diluted at 1:1000.
Blue: nuclear staining.
Zika Virus NS1 Antibody

ELISA: Zika Virus NS1 Antibody [NBP3-48633] -

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634158 as capture antibody at concentration of 5 ug/mL and NBP3-48633 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.
Zika Virus NS1 Antibody

Western Blot: Zika Virus NS1 Antibody [NBP3-48633] -

Non-infected (-) and infected (+) C6/36 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika Virus NS1 antibody (NBP3-48633) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Zika Virus NS1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Zika Virus NS1

Alternate Names

polyprotein, partial

Additional Zika Virus NS1 Products

Product Documents for Zika Virus NS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Zika Virus NS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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