ACE-2: The Receptor for SARS-CoV-2

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ACE-2 is an entry receptor for SARS-CoV-2, the virus responsible for coronavirus disease 19 (COVID-19). An Anti-ACE-2 Antibody from R&D Systems (Catalog # AF933) has been published to block entry of SARS-CoV-2 into cells expressing ACE-2. Learn more about strategies to detecting and blocking the SARS-CoV-2 and ACE-2 interactions.

 

What Is Coronavirus?

CoVs are a large family of enveloped, positive-sense, single-stranded RNA viruses that infect a broad range of vertebrates. They are extensive in bats but can be found in many other birds and mammals including humans. CoVs can cause a variety of diseases such as enteritis in pigs and cows and upper respiratory disease in chickens.1 In humans, CoVs tend to cause mild to moderate upper respiratory tract infections such as the common cold.2,3 However, in the past couple of decades, there have been outbreaks of severe, and sometimes fatal, respiratory illnesses that were later found to be caused by novel, human pathogenic CoVs. These CoV strains, which were determined to be phylogenetically distinct from the common human CoVs, had originated in bats and were transmitted to humans, typically through an intermediate host.1,4,5 These strains exhibited stronger virulence and quickly passed from human to human. While infection with these CoVs typically produced mild symptoms, for certain individuals, responses were more severe. In extreme cases, death occurred due to gradual respiratory failure as the result of alveolar damage.3,4,6

 

Comparing the SARS and COVID-19 Pandemics

The SARS pandemic that occurred in 2002 originated in the Guangdong Province in southern China. According to the World Health Organization (WHO), the CoV responsible for this disease, SARS-CoV, spread rapidly through 29 countries in Southeast Asia where it resulted in 8096 confirmed cases of SARS, with 774 of these individuals dying.7,8 In December 2019, a distinctive coronavirus (CoV) was determined to be responsible for an outbreak of potentially fatal atypical pneumonia, ultimately defined as coronavirus disease 19 (COVID-19), in Wuhan, Hubei province, China. This novel CoV, termed severe acute respiratory syndrome (SARS)-CoV-2, was found to be similar to the CoV that was responsible for the SARS pandemic that occurred in 2002. Today’s pandemic has surpassed these numbers.9 The rapid spread of this respiratory illness has resulted in a coordinated international effort to quickly characterize the etiology agent in order to develop potential vaccines and/or therapeutic agents that specifically target the virus or host cell components necessary for viral replication.

 

anti-ACE-2 antibody antibody attenuated SARS-CoV-2 entry into target cells
Figure 1. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2, the novel coronavirus responsible for coronavirus disease 2019 (COVID-19). Treatment with anti-ACE-2 antibodies disrupts the interaction between virus and receptor.

 

 

Identification and Characterization of SARS-CoV-2

Identification and sequencing of the virus responsible for COVID-19 (view SARS-CoV-2 protein sequence) determined that it was a novel CoV that shared 88% sequence identity with two bat-derived SARS-like CoV, suggesting it had originated in bats.10 Additionally, it was shown that this CoV, which was termed 2019-nCoV or SARS-CoV-2, shared 79.5% sequence identity with SARS-CoV.5,10 The coronaviral genome encodes four major structural proteins: the spike (S) protein, nucleocapsid (N) protein, membrane (M) protein, and the envelope (E) protein.6 The spike protein is responsible for facilitating entry of the CoV into the target cell. It is composed of a short intracellular tail, a transmembrane anchor, and a large ectodomain that consists of a receptor binding spike S1 subunit and a membrane-fusing spike S2 subunit.11 Sequence analysis of the SARS-CoV-2 spike protein genome showed that it was only 75% identical with the SARS-CoV spike protein.5,10 However, analysis of the receptor binding motif (RBM) in the spike protein showed that most of the amino acid residues essential for receptor binding were conserved between SARS-CoV and SARS-CoV-2, suggesting that the 2 CoV strains use the same host receptor for cell entry.12 The entry receptor utilized by SARS-CoV is Angiotensin-Converting Enzyme 2 (ACE-2).13

 

ACE-2

ACE-2 is a type I transmembrane metallocarboxypeptidase with homology to ACE, an enzyme long-known to be a key player in the Renin-Angiotensin system (RAS) and a target for the treatment of hypertension.14 It is mainly expressed in vascular endothelial cells, the renal tubular epithelium, and in Leydig cells in the testes.15,16 PCR analysis revealed that ACE-2 is also expressed in the lung, kidney, and gastrointestinal tract, tissues shown to harbor SARS-CoV.17-19 The major substrate for ACE-2 is Angiotensin II.20 ACE-2 degrades Angiotensin II to generate Angiotensin 1-7, thereby, negatively regulating RAS.15,20 ACE-2 has also been shown to exhibit a protective function in the cardiovascular system and other organs.15

 

ACE-2 is an Entry Receptor for SARS-CoV-2

Based on the sequence similarities of the RBM between SARS-CoV-2 and SARS-CoV, several independent research groups investigated if SARS-CoV-2 also utilizes ACE-2 as a cellular entry receptor. Zhou et al. showed that SARS-CoV-2 could use ACE-2 from humans, Chinese horseshoe bats, civet cats, and pigs to gain entry into ACE-2-expressing HeLa cells.5 Hoffmann et al. reported similar findings for human and bat ACE-2.21 Additionally, Hoffmann et al. showed that treating Vero-E6 cells, a monkey kidney cell line known to permit SARS-CoV replication, with an Anti-ACE-2 Antibody (R&D Systems, Catalog # AF933) blocked entry of VSV pseudotypes expressing the SARS-CoV-2 spike protein.21

 

Inhibiting TMPRSS2 Activity Blocks SARS-CoV-2 Entry

For SARS-CoV entry into a host cell, its spike protein needs to be cleaved by cellular proteases at 2 sites, termed spike protein priming, so the viral and cellular membranes can fuse.22 Specifically, spike protein priming by the serine protease TMPRSS2 is crucial for SARS-CoV infection of target cells and spread throughout the host.23,24 Hoffmann et al. investigated if SARS-CoV-2 entry is also dependent on spike protein priming by TMPRSS2. Treatment of the Calu-3 human lung cell line with the serine protease inhibitor camostat mesylate partially blocked entry of VSV pseudotypes expressing the SARS-CoV-2 spike protein.21 Similar effects of camostat mesylate treatment were seen with primary human lung cells and with Calu-3 cells incubated with authentic SARS-CoV-2.21

 

Avenues for COVID-19 Therapies

These new findings could greatly impact the development of effective therapies for COVID-19. For instance, anti-ACE-2 antibodies could be used to block SARS-CoV-2 binding to the receptor. Additionally, TMPRSS2 inhibitors could be used to prevent SARS-CoV-2 entry into host cells. Camostat has been used to treat chronic pancreatitis in Japan and is currently undergoing Phase 1/2 trial testing in the United States.26 If deemed safe, camostat could be a potential treatment option of CoV infections.27 It is also possible that antibodies developed during SARS-CoV infection could help prevent or treat COVID-19. Hoffmann et al. showed that sera from recovering SARS patients reduced SARS-CoV-2 spike protein-driven entry into Vero-E6 cells.21 However, future studies are needed to determine whether any of these options are effective in disrupting the interaction between virus and receptor in vivo.

 

References

  1. Fehr, A.R. and S. Perlman (2015) Methods Mol. Biol. 1282:1.
  2. Centers for Disease Control and Prevention (n.d.) Common Human Coronaviruses. https://www.cdc.gov/coronavirus/general-information.html.
  3. Yang, Y. et al. (2020) J. Autoimmun. [Epub ahead of print].
  4. Gu, J. and C. Korteweg (2007) Am. J. Pathol. 170:1136.
  5. Zhou, P. et al. (2020) Nature [Epub ahead of print].
  6. Schoeman, D. and B.C. Fielding (2019) Virol. J. 16:69.
  7. de Wit, E. et al. (2016) Nat. Rev. Microbiol. 14:523.
  8. WHO (2003) Summary of Probable SARS Cases with Onset of Illness from 1 November 2002 to 31 July 2003.
  9. WHO (2020) Coronavirus Disease 2019 (COVID-10) Situation Report – 50.
  10. Lu, R. et al. (2020) Lancet. 395:565.
  11. Li, F. (2016) Annu. Rev. Virol. 3:237.
  12. Wan, Y. et al. (2020) J. Virol. [Epub ahead of print].
  13. Li, W. et al. (2003) Nature 426:450.
  14. Riordan, J.F. (2003) Genome Bio. 4:225.
  15. Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
  16. Jinag, F. et al. (2014) Nat. Rev. Cardiol. 11:413.
  17. Ksiazek, T.G. et al. (2003) N. Engl. J. Med. 348:1953.
  18. Harmer, D. et al. (2002) FEBS Lett. 532:107.
  19. Leung, W.K. et al. (2003) Gastroenterology 125:1011.
  20. Tikellis, C. and M.C. Thomas (2012) Int. J. Pept. 2012:256294.
  21. Hoffmann, M. et al. (2020) Cell [Epub ahead of print].
  22. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:5871.
  23. Matsuyama, S. et al. (2010) J. Virol. 84:12658.
  24. Iwata-Yoshikawa, N. et al. (2019) J. Virol. 93:e01815.
  25. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  26. Ramsey, M.L. et al. (2019) Trials 20:501.
  27. Zhou, Y. et al. (2015) Antiviral Res. 116:76.