Citations: Recombinant Proteins: Neuronal Outgrowth, Survival, and Guidance

R&D Systems offers a wide selection of active recombinant proteins to facilitate study of the mechanisms that regulate the growth and guidance of axons. These include classical axon guidance cues such as Netrins, Semaphorins, Ephrins, cell adhesion mole­cules, and their corresponding receptors. Also available are a range of tools to study regulators of axon outgrowth and survival including mem­bers of the neurotrophin family, their receptors, co-receptors, and myelin-associated proteins. Several recent studies have utilized R&D Systems products to assess the roles of these factors during development, regeneration, and neurode­generative disease.

  1. Mouse Ephrin-A1/Fc (Catalog # 602-A1)
    Human Ephrin-B3/Fc (Catalog # 395-EB)

    Egea, J. et al. (2005) Regulation of EphA 4 kinase activity is required for a subset of axon guidance decisions suggesting a key role for receptor clustering in Eph function. Neuron 47:515.
    Sample Type: Mouse Cortical Neurons
  2. Mouse Ephrin-B1/Fc (Catalog # 473-EB)
    Mouse Ephrin-A1/Fc (Catalog # 602-A1)
    Human Ephrin-A5/Fc (Catalog # 374-EA)

    Himanen, J. -P. et al. (2004) Repelling class discrim­ination: ephrin-A5 binds to and activates EphB2 recep­tor signaling. Nat. Neurosci. 7:501.
    Sample Type: COS-1 Cells (African green monkey kidney)
  3. Mouse Netrin-1 (Catalog # 1109-N1)
    Human BDNF (Catalog # 248-BD)

    Jin, M. et al. (2005) Ca2+-dependent regulation of Rho GTPases triggers turning of nerve growth cones.
    J. Neurosci. 25:2338.
    Sample Type: Xenopus Neurons, Mouse Cerebellar Granule Cells, Mouse Cortical Neurons
  4. Chicken Netrin-1 (Catalog # 128-N1)
    Liu, G. et al. (2004) Netrin requires focal adhesion kinase and Src family kinases for axon outgrowth and attraction. Nat. Neurosci. 7:1222.
    Sample Type: Rat Cortical Neurons
  5. Human NT-3 (Catalog # 267-N3)
    Human NT-4 (Catalog # 268-N4)
    Human BDNF (Catalog # 248-BD)

    Banin, E. et al. (2006) Retinal incorporation and dif­ferentiation of neuronal precursors derived from embryonic stem cells. Stem Cells 24:246.
    Sample Type: Human Embryonic Stem Cells
  6. Human TrkA/Fc (Catalog # 175-TK)
    Human NGF R/p75NTR (Catalog # 367-NR)

    Nykjaer, A. et al. (2004) Sortilin is essential for proNGF-induced neuronal cell death. Nature 427:823.
    Sample Type: In vitro binding to human NGF and human Pro-NGF
  7. Human b-NGF (Catalog # 256-GF)
    Human BDNF (Catalog # 248-BD)
    Human NT-4 (Catalog # 268-N4)
    Rat MAG/Fc (Catalog # 538-MG)

    Williams, G. et al. (2005) Overcoming inhibitors of myelin with a novel neurotrophin strategy. J. Biol. Chem. 280:5862.
    Sample Type: Rat Cerebellar Neurons
  8. Rat MAG/Fc (Catalog # 538-MG)
    Mouse OMgp (Catalog # 1674-MG)

    Kim, J. -E. (2004) Nogo-66 receptor prevents raphespinal and rubrospinal axon regeneration and limits functional recovery from spinal cord injury. Neuron 44:439.
    Sample Type: Mouse DRG Neurons
  9. Rat Neuropilin-1/Fc (Catalog # 566-NNS)
    Rat Neuropilin-2/Fc (Catalog # 567-N2)

    Anderson, C.N.G. et al. (2003) Molecular analysis of axon repulsion by the notochord. Development 130:1123.
    Sample Type: Chick Notochord-expressed Semaphorin
  10. Human NCAM-L1/Fc (Catalog # 777-NC)
    Maretzky, T. et al. (2005) L1 is sequentially processed by two differently activated metalloproteases and presenilin/g-secretase and regulates neural cell adhesion, cell migration, and neurite outgrowth.
    Mol. Cell. Biol. 25:9040.
    Sample Type: Mouse Cerebellar Explants