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Organelles: Autophagosomes

Property title
ATG3
ATG3
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ATG4A
ATG4A
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ATG4B
ATG4B
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ATG5
ATG5
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ATG7
ATG7
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ATG12
ATG12
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LC3/
MAP1LC3A
LC3/
MAP1LC3A
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p62/SQSTM1
p62/SQSTM1
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Overview

Autophagosomes are double membrane vesicles that form in response to cellular stress. The process of autophagy refers to the destruction of cytoplasmic protein aggregates or defective cellular organelles by autophagosome fusion with lysosomes.

Data Examples

LC3A in Human Brain Cortex Tissue. LC3A was detected in immersion fixed paraffin-embedded sections of human brain cortex tissue using Rat Anti-Human LC3A Monoclonal Antibody (Catalog # MAB8558) at 15 µg/mL overnight at 4°C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p62/SQSTM1 in HeLa Human Cell Line. p62/SQSTM1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat p62/SQSTM1 Monoclonal Antibody (Catalog # MAB8028) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to phagosomes in cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ATG4A in RAW 264.7 Mouse Cell Line. ATG4A was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG4A Monoclonal Antibody (Catalog # MAB4324) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.