Subtype Specific T Cell Enrichment Columns Assay Principle

For purification of specific T cell subpopulations by high affinity negative selection.

Kits designed to isolate specific T cell subtypes employ an initial antibody incubation step. Undesired T cell populations are targeted using a cocktail of monoclonal antibodies.

Samples are subsequently loaded onto enrichment columns containing glass beads coated with Ig and anti-Ig. Following simple gravitational flow, B cells, monocytes and the undesired T cells are removed from the sample by high affinity negative selection.