A Guide to the Colony Forming Cell Assay: Methods and Tips

Joy Aho, PhD., Manager of Stem Cells Research and Development

This video protocol provides step-by-step instructions on how to consistently perform the Colony Forming Cell (CFC) Assay. Tips are provided throughout the video to help optimize the assay procedure, including tricks to accurately evaluate and score colony formations.

  • 00:10 CFC Assay Overview
  • 00:54 Aliquoting the Media
  • 02:06 Preparing the Cells
  • 04:05 Plating the Cells
  • 05:14 Counting the Cells
  • 05:53 Tips for Colony Identification

Full Written protocols for our CFC Assays:

The Human Colony Forming Cell (CFC) Assay

The Mouse/Rat Colony Forming Cell (CFC) Assay

The Mouse Pre-B Colony Forming Cell (CFC) Assay

Guides to choosing methylcellulose media:

For the Human CFC Assay

For the Mouse CFC Assay

For the Rat CFC Assay

Recorded on August 26, 2014