Chinese Hamster Ovary cell line, CHO-derived, Ala279-Ser390
N-terminal Sequence Analysis:
<0.10 EU per 1 μg of the protein by the LAL method.
>97%, by SDS-PAGE with silver staining.
Predicted Molecular Mass:
12.8 kDa (monomer)
12 kDa, reducing conditions
24 kDa, non-reducing conditions
Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is typically 0.04-0.2 ng/mL.