Products

Recombinant Human TGF‑beta 1

DESCRIPTION
CATALOG #
SIZEPRICEQTY 
Recombinant Human TGF-beta 1
240-B-010 10 µg $809
Recombinant Human TGF-beta 1
240-B-002 2 µg $259
Recombinant Human TGF-beta 1, CF
240-B-001MG/CF 1 mg $13,500
Recombinant Human TGF-beta 1, CF
240-B-010/CF 10 µg $809
Recombinant Human TGF-beta 1, CF
240-B-002/CF 2 µg $259
Recombinant Human TGF‑beta 1 BulkGet Quote Bulk/Custom

Source: Chinese Hamster Ovary cell line, CHO-derived, Ala279-Ser390
Accession #: P01137
N-terminal Sequence Analysis: Ala279
Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE with silver staining.
Predicted Molecular Mass: 12.8 kDa (monomer)
SDS-PAGE: 12 kDa, reducing conditions
24 kDa, non-reducing conditions
Activity: Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is typically 0.04-0.2 ng/mL.

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

Which formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

240-B 240-B/CF

Formulation: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details.

Formulation: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details.

Reconstitution: Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute at 20 µg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.

Reconstitution: Reconstitute 2 µg vials at 20 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. Reconstitute 10 µg or larger vials at 100 µg/mL in sterile 4 mM HCl.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

DATA

SDS-PAGE Recombinant Human TGF-beta 1
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SDS-PAGE: 1 ug/lane of Recombinant Human TGF-beta 1 was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.

Recombinant Human/Mouse/Rat Activin A
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Bioactivity: Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is typically 0.04-0.2 ng/mL.

Recombinant  Human/Mouse/Rat Activin A Promotes the Differentiation of Pluripotent Stem  Cells into Endoderm.
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Recombinant Human TGF-beta 1 Induces Epithelial to Mesenchymal Transition. Epithelial to Mesenchymal Transition (EMT) was induced in the A549 human lung carcinoma cell line with cell culture media supplemented with Recombinant Human (rh)TGF-beta 1 (Catalog # 240-B). Control cells were cultured without rhTGF-beta 1. EMT induction was confirmed at 48 h by flow cytometric staining for E-Cadherin (filled; Catalog # FAB18381P), an epithelial cell marker, or an isotype control (open; Catalog # IC0041P). TGF-beta 1 decreased the expression of E-Cadherin.