Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Formulations:
Catalog # Availability Size / Price Qty
7754-BH-005
7754-BH-025
7754-BH-100
7754-BH-500
7754-BH-01M
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (10)

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human TGF-beta 1 protein
Ala279-Ser390
Accession #
N-terminal Sequence
Analysis
Ala279
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.8 kDa (monomer)
SDS-PAGE
11 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7754-BH

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7754-BH/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 1

TGF‑ beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, ‑2 and ‑3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta  RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

References
  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press p. 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name
Transforming Growth Factor beta 1
Entrez Gene IDs
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names
CEDLAP; DPD1; latency-associated peptide; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Citations for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Retinoic acid signaling is essential for airway smooth muscle homeostasis
    Authors: F Chen, F Shao, A Hinds, S Yao, S Ram-Mohan, TA Norman, R Krishnan, A Fine
    JCI Insight, 2018;3(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Ruxolitinib in combination with prednisone and nilotinib exhibit synergistic effects in human cells lines and primary cells from myeloproliferative neoplasms
    Authors: A Arenas Cor, R Ayala Diaz, P Hernández-, J Gorrochate, D Primo, A Robles, ML Morales, J Ballestero, I Rapado, M Gallardo, M Linares, J Martínez-L
    Haematologica, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association
    Authors: H Huang, S Liu, M Jean, S Simpson, H Huang, M Merkley, T Hayashi, W Kong, I Rodríguez-, X Zhang, HO Yosief, H Miao, J Que, JJ Kobie, J Bradner, NG Santoso, W Zhang, J Zhu
    Front Microbiol, 2017;8(0):1035.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Decoding NADPH oxidase 4 expression in human tumors
    Authors: JL Meitzler, HR Makhlouf, S Antony, Y Wu, D Butcher, G Jiang, A Juhasz, J Lu, I Dahan, P Jansen-Dür, H Pircher, AM Shah, K Roy, JH Doroshow
    Redox Biol, 2017;13(0):182-195.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    • TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Average Rating: 4.9 (Based on 10 Reviews)

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Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 08/30/2019
Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 05/07/2019
Application: Binding assay/Protein-protein interaction

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 02/10/2019
Application: Immunoassay Standard

TGFB1 human peritoneal mesothelial cells


Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 05/22/2018
Application: CellProlif

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 04/12/2018
Application: Cell migration/motility

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 03/08/2018
Application: CellProlif

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 03/07/2018
Application: Enzymatic activity in vitro

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 02/17/2018
Application: Apoptosis assay

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 02/14/2018
Application: CellProlif

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 10/01/2017
Application: Stem/Immune cell maintenance or differentiation