Technical Information

IHC Images

IHC/ICC: CEACAM-1/CD66a

CEACAM-1 in Human Colon.
CEACAM-1 in Human Colon.

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM-1) was detected in immersion fixed paraffin-embedded sections of human colon using Human CEACAM-1 Monoclonal Antibody (Catalog # MAB22441) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

This antibody specifically recognizes human CEACAM-1. In ELISA and Western blot experiments, this antibody does not cross-react with recombinant human CD31, recombinant human ICAM-1, -2, -3, recombinant mouse MAdCAM-1, or recombinant human VCAM-1.

Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.


CEACAM-1/CD66a in Human Colon Cancer Tissue.
CEACAM-1/CD66a in Human Colon Cancer Tissue.

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1, Hepatitis B (CEACAM-1/CD66a) was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Human CEACAM-1/CD66a Monoclonal Antibody (Catalog # MAB22441) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.


CEACAM-1/CD66a in Mouse Liver.
CEACAM-1/CD66a in Mouse Liver.

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1, Hepatitis B (CEACAM-1/CD66a) was detected in perfusion fixed frozen sections of mouse liver using Sheep Anti-Mouse CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.


CEACAM-1/CD66a in Mouse Liver.
CEACAM-1/CD66a in Mouse Liver.

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1, Hepatitis B (CEACAM-1/CD66a) was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse CEACAM-1/CD66a Monoclonal Antibody (Catalog # MAB6480) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to bile canaliculi. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.