Mouse IgG HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
HAF018
Detection of MouseIgG Primary Antibody by Western Blot
1 Image
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews

Mouse IgG HRP-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Supplied as a 0.2 μm filtered solution in PBS with stabilizers.
Label
HRP (Horseradish Peroxidase)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of MouseIgG Primary Antibody by Western Blot View Larger

Detection of MouseIgG Primary Antibody by Western Blot Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4Monoclonal Antibody (Catalog # MAB41642)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # HAF018).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Mouse IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

  1. Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
    Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
    Nat Commun, 2017;8(1):552.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study
    Authors: R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa
    Cell Death Dis, 2017;8(8):e3017.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: WB
  3. Heterozygous Vangl2(Looptail) mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair
    Authors: T Poobalasin, LL Yates, SA Walker, M Pereira, NY Gross, A Ali, M Kolatsi-Jo, MR Jarvelin, J Pekkanen, E Papakrivop, DA Long, M Griffiths, D Wagner, M Königshoff, M Hind, C Minelli, CM Lloyd, CH Dean
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  4. Polysialic acid regulates sympathetic outflow by facilitating information transfer within the nucleus of the solitary tract
    Authors: P Bokiniec, S Shahbazian, SJ McDougall, BA Berning, D Cheng, IJ Llewellyn-, PG Burke, S McMullan, M Mühlenhoff, H Hildebrand, F Braet, M Connor, NH Packer, AK Goodchild
    J. Neurosci., 2017;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Functional Assay
  5. mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress
    Authors: Y Xia, M Sun, Y Xie, R Shu
    Oxid Med Cell Longev, 2017;2017(0):6292630.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  6. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target
    Sci Rep, 2016;6(0):37360.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  7. Altered mitochondrial function and energy metabolism is associated with a radioresistant phenotype in oesophageal adenocarcinoma.
    Authors: Lynam-Lennon, Niamh, Maher, Stephen, Maguire, Aoife, Phelan, James, Muldoon, Cian, Reynolds, John V, O'Sullivan, Jacintha
    PLoS ONE, 2014;9(6):e100738.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  8. Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin.
    Authors: Krishnatry AS, Brazeau DA, Fung HL
    Nitric Oxide, 2010;22(1):11-7.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: WB
  9. Sleeping beauty transposition from nonintegrating lentivirus.
    Authors: Vink CA, Gaspar HB, Gabriel R, Schmidt M, McIvor RS, Thrasher AJ, Qasim W
    Mol. Ther., 2009;17(7):1197-204.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
View all Antibody FAQs

Reviews for Mouse IgG HRP-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse IgG HRP-conjugated Antibody and earn rewards!

Have you used Mouse IgG HRP-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review