Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.
Polyclonal Donkey IgG
Supplied as a 0.2 μm filtered solution in PBS with stabilizers.
HRP (Horseradish Peroxidase)
Use at a 1:1000 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of MouseIgG Primary Antibody by Western Blot Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4Monoclonal Antibody (Catalog # MAB41642)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # HAF018). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
6 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.