Mouse IgG HRP-conjugated Antibody

  (9 citations)     
Datasheet / CoA / SDS
Product Details
Citations (9)
FAQs
Supplemental Products
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.
  • Source
    Polyclonal Donkey IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse IgG
  • Formulation
    Supplied as a 0.2 μm filtered solution in PBS with stabilizers.
  • Label
    HRP (Horseradish Peroxidase)
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Use at a 1:1000 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of MouseIgG Primary Antibody by Western Blot Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4Monoclonal Antibody (Catalog # MAB41642)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # HAF018).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Heterozygous Vangl2(Looptail) mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair
    Authors: T Poobalasin, LL Yates, SA Walker, M Pereira, NY Gross, A Ali, M Kolatsi-Jo, MR Jarvelin, J Pekkanen, E Papakrivop, DA Long, M Griffiths, D Wagner, M Königshoff, M Hind, C Minelli, CM Lloyd, CH Dean
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress
    Authors: Y Xia, M Sun, Y Xie, R Shu
    Oxid Med Cell Longev, 2017;2017(0):6292630.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study
    Authors: R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa
    Cell Death Dis, 2017;8(8):e3017.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  4. Polysialic acid regulates sympathetic outflow by facilitating information transfer within the nucleus of the solitary tract
    Authors: P Bokiniec, S Shahbazian, SJ McDougall, BA Berning, D Cheng, IJ Llewellyn-, PG Burke, S McMullan, M Mühlenhoff, H Hildebrand, F Braet, M Connor, NH Packer, AK Goodchild
    J. Neurosci., 2017;0(0):.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: Functional Assay
  5. Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
    Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
    Nat Commun, 2017;8(1):552.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target
    Sci Rep, 2016;6(0):37360.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Altered mitochondrial function and energy metabolism is associated with a radioresistant phenotype in oesophageal adenocarcinoma.
    Authors: Lynam-Lennon, Niamh, Maher, Stephen, Maguire, Aoife, Phelan, James, Muldoon, Cian, Reynolds, John V, O'Sullivan, Jacintha
    PLoS ONE, 2014;9(6):e100738.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin.
    Authors: Krishnatry AS, Brazeau DA, Fung HL
    Nitric Oxide, 2010;22(1):11-7.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  9. Sleeping beauty transposition from nonintegrating lentivirus.
    Authors: Vink CA, Gaspar HB, Gabriel R, Schmidt M, McIvor RS, Thrasher AJ, Qasim W
    Mol. Ther., 2009;17(7):1197-204.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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