Mouse IgG HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse IgG Primary Antibody by Western Blot
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Product Details
Citations (13)
Supplemental Products
Reviews (2)

Mouse IgG HRP-conjugated Antibody Summary

Species Reactivity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.
Polyclonal Donkey IgG
Antigen Affinity-purified
Mouse IgG
Supplied as a 0.2 μm filtered solution in PBS with stabilizers.
HRP (Horseradish Peroxidase)(Excitation= nm, Emission= nm)


Recommended Concentration
Western Blot
Use at a 1:1000 dilution.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IgG Primary Antibody by Western Blot View Larger

Detection of Mouse IgG Primary Antibody by Western Blot Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB41642) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Mouse IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Leishmania donovani Impedes Antileishmanial Immunity by Suppressing Dendritic Cells via the TIM-3 Receptor
    Authors: MN Akhtar, S Kumar, P Sen
    MBio, 2022-08-04;13(4):e0330921.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression System
    Authors: A Tarar, EM Alyami, CA Peng
    Methods and protocols, 2020-12-01;3(4):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  3. 2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy
    Authors: A Singhal, ES Krystofiak, WG Jerome, B Song
    Sci Rep, 2020-05-26;10(1):8663.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. 2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia
    Authors: E Tkaczynski, A Arulselvan, J Tkaczynski, S Avery, L Xiao, B Torok-Stor, K Abrams, NV Rao, G Johnson, TP Kennedy, M Poncz, MP Lambert
    Blood Adv, 2018-04-10;2(7):754-761.
    Species: Mouse
    Sample Types: Protein
    Applications: ELISA Capture
  5. Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
    Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
    Nat Commun, 2017-09-15;8(1):552.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study
    Authors: R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa
    Cell Death Dis, 2017-08-24;8(8):e3017.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress
    Authors: Y Xia, M Sun, Y Xie, R Shu
    Oxid Med Cell Longev, 2017-07-19;2017(0):6292630.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Polysialic acid regulates sympathetic outflow by facilitating information transfer within the nucleus of the solitary tract
    Authors: P Bokiniec, S Shahbazian, SJ McDougall, BA Berning, D Cheng, IJ Llewellyn-, PG Burke, S McMullan, M Mühlenhoff, H Hildebrand, F Braet, M Connor, NH Packer, AK Goodchild
    J. Neurosci., 2017-06-02;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Functional Assay
  9. Heterozygous Vangl2(Looptail) mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair
    Authors: T Poobalasin, LL Yates, SA Walker, M Pereira, NY Gross, A Ali, M Kolatsi-Jo, MR Jarvelin, J Pekkanen, E Papakrivop, DA Long, M Griffiths, D Wagner, M Königshoff, M Hind, C Minelli, CM Lloyd, CH Dean
    Dis Model Mech, 2017-02-24;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target
    Sci Rep, 2016-11-17;6(0):37360.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Altered mitochondrial function and energy metabolism is associated with a radioresistant phenotype in oesophageal adenocarcinoma.
    Authors: Lynam-Lennon, Niamh, Maher, Stephen, Maguire, Aoife, Phelan, James, Muldoon, Cian, Reynolds, John V, O'Sullivan, Jacintha
    PLoS ONE, 2014-06-26;9(6):e100738.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin.
    Authors: Krishnatry AS, Brazeau DA, Fung HL
    Nitric Oxide, 2009-10-15;22(1):11-7.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  13. Sleeping beauty transposition from nonintegrating lentivirus.
    Authors: Vink CA, Gaspar HB, Gabriel R, Schmidt M, McIvor RS, Thrasher AJ, Qasim W
    Mol. Ther., 2009-05-05;17(7):1197-204.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Mouse IgG HRP-conjugated Antibody
By Anonymous on 11/27/2021
Application: WB Sample Tested: Colon tissue,Tissue Lysates Species: Mouse

Mouse IgG HRP-conjugated Antibody
By Anonymous on 08/05/2020
Application: WB Sample Tested: HeLa cells,HUVEC human umbilical vein endothelial cells Species: Human