Human PP14/Glycodelin Antibody

Catalog # Availability Size / Price Qty
MAB842-100
MAB842-SP

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Detection of Human PP14/Glycodelin by Western Blot.
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Human PP14/Glycodelin Antibody Summary

Species Reactivity
Human
Specificity
Detects human PP14/Glycodelin in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 929718
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PP14/Glycodelin
Met19-Phe180
Accession # P09466
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human PP14/Glycodelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF842).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PP14/Glycodelin antibody by Western Blot. View Larger

Detection of Human PP14/Glycodelin by Western Blot. Western blot shows lysates of human uterus tissue and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PP14/Glycodelin Monoclonal Antibody (Catalog # MAB842) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PP14/Glycodelin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP14/Glycodelin

Placenta Protein 14 (PP14, also known as Glycodelin-A) is a 28-kDa glycoprotein that is mainly expressed by late secretory-phase endometrium and first-trimester gestational endometrium. It is present at high levels in amniotic fluid, and in the serum of pregnant women. PP14 has been reported to have immuno- suppressive activity that may play a role in fetal survival during pregnancy.

Long Name
Placental Protein 14
Entrez Gene IDs
5047 (Human)
Alternate Names
alpha uterine protein; GdF; GdS; Glycodelin; glycodelin-A; glycodelin-F; glycodelin-S; MGC138509; MGC142288; PAEP; PEP; Placental protein 14; PP14 protein (placental protein 14); PP14; PP14GDPAEGGdA; pregnancy-associated endometrial a; Pregnancy-associated endometrial alpha-2 globulin; pregnancy-associated endometrial alpha-2-globulin; progestagen-associated endometrial protein (placental protein 14; progestagen-associated endometrial proteinPEG; Progesterone-associated endometrial protein

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