DNA Damage, Mutation Detection and Enzyme Kits/Activity Assays
- DNA Damage Detection
- DNA Repair
- Mismatch Repair Kits
- Oligonucleotide Labeling Kit
- Enzyme Activity Assays
CometAssay™
Single Cell Gel Electrophoresis Assay
Catalog #: TA800,TA4251
Label: SYBR Green Dye Silver Stain
Description: Cells are immobilized on a slide in a bed of low melting point agarose and then gently lysed. Following lysis, samples are treated with alkali to unwind and denature the DNA and hydrolyze sites of damage. The samples are then electrophoresed, stained, and visualized by microscopy. Evidence of a "comet tail" shape and migration pattern indicates DNA Damage.
Testing Format: Fluorescence and light microscopy
Sample Source: Unfixed cells, Fresh tissues
FLARE™ Assay
Fragment Length Analysis using Repair Enzymes
The FLARE assay kits provide the reagents to detect certain types of DNA damage by using specific enzymes in conjunction with the CometAssay. The type of DNA damage is inferred from the substrate specificity of the enzyme used in the assay.
Human DNA Polymerase ß Kit
DNA Repair Enzyme Kit
Catalog #: 4020-100-K
Unit Definition: One unit is the amount of enzyme required to catalyze the incorporation of 1nmole of dNTP into an acid-insoluble form in 60 minutes at 37°C.
Kit Contents:
- Human DNA Polymerase ß
- ß-polymerase Control DNA
- 10X REC™ Buffer
- 5X REC Loading Buffer
- REC Water
- 1 mM aphidicolin
E. coli Endonuclease V Kit
Mismatch Repair Enzyme Kit
Catalog #: 4035-500-K
Unit Definition: One unit is the amount of enzyme required to cleave 1 pmole of a 32P-labeled oligonucleotide probe in one hour at 37° C at a C/C or A/A mismatch when hybridized to a complementary oligonucleotide.
Kit Contents:
- Endonuclease V
- 10X REC Buffer 2
- REC Water
- 3X Alkaline Loading Buffer
- MUA Oligonucleotide
- MLA Oligonucleotide
- MLT Oligonucleotide
E. coli MutY DNA Glycosylase Kit
Mismatch Repair Enzyme Kit
Catalog #: 4000-500-K
Unit Definition: One unit is the amount of enzyme required to cleave 1 pmole of an oligonucleotide duplex containing an A/G mismatch in 1 hour at 37° C. Only the strand with the A is cleaved.
Kit Contents:
- Mut Y
- 10X REC Buffer 4
- REC Water
- 3X Alkaline Loading Buffer
- MUA Oligonucleotide
- MLA Oligonucleotide
- MLT Oligonucleotide/li
OligoTag™ Kinase Kit
Oligonucleotide Labeling Kit
Catalog #: 9500-25-K
Description: The kit provides all the necessary reagents (except labeled nucleotide) to generate a high specific activity 5' end labeled DNA sequence.
Kit Contents:
- 10X Kinase Buffer
- 10 mM ATP
- Deionized Water
- Control Oligonucleotide
- T4 Polynucleotide Kinase
PARP Universal Colorimetric Assay
Enzymatic Activity Assay
Catalog #: 4672-096-K
Label: TACS-Sapphire™
Description: For the measurement of poly (ADP-ribos) polymerase (PARP) activity, and for the screening of PARP inhibitors.
Format: Colorimetric Assay
Samples: Unfixed Cells, Fresh Tissues
Superoxide Dismutase Assay (SOD)
Enzymatic Activity Assay
Catalog #: 7500-100-K
Description: Superoxide dismutase (SOD) catalyzes the dismutation of the superoxide radical (02-) into hydrogen peroxide (H2O2) and elemental oxygen (O2). Contains 50 positive and 50 negative control tests.
Features:
- Suitable for the assay of (Mn2+)-SOD, (Fe2+)-SOD, and (Cu/Zn)-SOD
- Each sample takes only 5 minutes.
- Ideal for assaying SOD in mammalian cell lysates.
- Not greatly disturbed by trace metals.
Kit Contents:
- SOD (1 unit/µL)
- 25X SOD Reaction Buffer
- Xanthine Solution
- NBT Solution
- XOD Solution
- 20X Cell Lysis Solution