DNA Damage, Mutation Detection, and Enzyme Kits/Activity Assays

    CometAssay®

    Single Cell Gel Electrophoresis Assay

    Catalog #: 4250-050-K, 4251-050-K
    Label: SYBR Green Dye Silver Stain
    Description: Cells are immobilized on a slide in a bed of low melting point agarose and then gently lysed. Following lysis, samples are treated with alkali to unwind and denature the DNA and hydrolyze sites of damage. The samples are then electrophoresed, stained, and visualized by microscopy. Evidence of a "comet tail" shape and migration pattern indicates DNA Damage.
    Testing Format: Fluorescence and light microscopy
    Sample Source: Unfixed cells, Fresh tissues

    FLARE™ Assay

    Fragment Length Analysis using Repair Enzymes

    The FLARE assay kits provide the reagents to detect certain types of DNA damage by using specific enzymes in conjunction with the CometAssay. The type of DNA damage is inferred from the substrate specificity of the enzyme used in the assay.
    Catalog # Tests
    Human OGG1 4130-100-FK 75 Tests
    Chorella virus PDG 4065-100-FK 75 Tests
    Endonuclease III 4045-100-FK 75 Tests
    Fpg 4040-100-FK 75 Tests
    T4-Endonuclease V 4055-100-FK 75 Tests
    UVDE 4100-100-FK 75 Tests
    FLARE Slides 3950-075-02 25 Each
    3950-300-02 100 Each

    PARP Universal Colorimetric Assay

    Enzymatic Activity Assay

    Catalog #: 4677-096-K
    Label: TACS-Sapphire™
    Description: For the measurement of poly (ADP-ribos) polymerase (PARP) activity, and for the screening of PARP inhibitors.
    Format: Colorimetric Assay
    Samples: Unfixed Cells, Fresh Tissues

    Superoxide Dismutase Assay (SOD)

    Enzymatic Activity Assay

    Catalog #: 7500-100-K
    Description: Superoxide dismutase (SOD) catalyzes the dismutation of the superoxide radical (02-) into hydrogen peroxide (H2O2) and elemental oxygen (O2). Contains 50 positive and 50 negative control tests.
    Features:
    • Suitable for the assay of (Mn2+)-SOD, (Fe2+)-SOD, and (Cu/Zn)-SOD
    • Each sample takes only 5 minutes.
    • Ideal for assaying SOD in mammalian cell lysates.
    • Not greatly disturbed by trace metals.
    Kit Contents:
    • SOD (1 unit/µL)
    • 25X SOD Reaction Buffer
    • Xanthine Solution
    • NBT Solution
    • XOD Solution
    • 20X Cell Lysis Solution