CometAssay®
Single Cell Gel Electrophoresis Assay
Catalog #: 4250-050-K, 4251-050-K
Label: SYBR Green Dye Silver Stain
Description: Cells are immobilized on a slide in a bed of low melting point agarose and then gently lysed. Following lysis, samples are treated with alkali to unwind and denature the DNA and hydrolyze sites of damage. The samples are then electrophoresed, stained, and visualized by microscopy. Evidence of a "comet tail" shape and migration pattern indicates DNA Damage.
Testing Format: Fluorescence and light microscopy
Sample Source: Unfixed cells, Fresh tissues
FLARE™ Assay
Fragment Length Analysis using Repair Enzymes
The FLARE assay kits provide the reagents to detect certain types of DNA damage by using specific enzymes in conjunction with the CometAssay. The type of DNA damage is inferred from the substrate specificity of the enzyme used in the assay.
PARP Universal Colorimetric Assay
Enzymatic Activity Assay
Catalog #: 4677-096-K
Label: TACS-Sapphire™
Description: For the measurement of poly (ADP-ribos) polymerase (PARP) activity, and for the screening of PARP inhibitors.
Format: Colorimetric Assay
Samples: Unfixed Cells, Fresh Tissues
Superoxide Dismutase Assay (SOD)
Enzymatic Activity Assay
Catalog #: 7500-100-K
Description: Superoxide dismutase (SOD) catalyzes the dismutation of the superoxide radical (02-) into hydrogen peroxide (H2O2) and elemental oxygen (O2). Contains 50 positive and 50 negative control tests.
Features:
- Suitable for the assay of (Mn2+)-SOD, (Fe2+)-SOD, and (Cu/Zn)-SOD
- Each sample takes only 5 minutes.
- Ideal for assaying SOD in mammalian cell lysates.
- Not greatly disturbed by trace metals.
Kit Contents:
- SOD (1 unit/µL)
- 25X SOD Reaction Buffer
- Xanthine Solution
- NBT Solution
- XOD Solution
- 20X Cell Lysis Solution