14-3-3 epsilon Antibody (CPTC-YWHAE-1)

Novus Biologicals | Catalog # NBP2-79810

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # CPTC-YWHAE-1
View all 14-3-3 epsilon Primary Antibodies »

Product Specifications

Immunogen

Recombinant human full-length 14-3-3 epsilon protein (Uniprot: P62268)

Localization

Cytoplasm, Melanosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.

Antibody with azide - store at 2 to 8C.

Scientific Data Images for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)

Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]

Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]

Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3 epsilon Antibody (CPTC-YWHAE-1).
Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Formalin-fixed, paraffin-embedded human Testis stained with 14-3-3 epsilon Antibody (CPTC-YWHAE-1).
Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]

Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]

Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. 14-3-3 epsilon Antibody (NBP2-79810) primary antibody at 1:1000, 4C, overnight. Wb image submitted by a verified customer review.
Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]

Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]

Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Analysis of Protein Array containing more than 19,000 full-length human proteins using 14-3-3 epsilon Antibody (CPTC-YWHAE-1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-79810 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: 14-3-3 epsilon

14-3-3 epsilon product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Alternate Names

1433 epsilon, YWHAE

Gene Symbol

YWHAE

UniProt

P62268 (Rat)

Additional 14-3-3 epsilon Products

Product Documents for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)

Certificate of Analysis

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Product Specific Notices for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for 14-3-3 epsilon Antibody (CPTC-YWHAE-1) (1)

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  • 14-3-3 epsilon Antibody (CPTC-YWHAE-1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: BREAST CANCER CELL LINES, Sample Amount: 40 ug
    Species: Human
    Verified Customer | Posted 03/06/2021
    Western Blot: MDA-MB-231 and MCF-7 whole cell lysates were loaded with 40 ug/lane. 10% SDS-PAGE. 14-3-3 epsilon Antibody (NBP2-79810) primary antibody: 1:1000, 4℃, overnight.  
    14-3-3 epsilon Antibody (CPTC-YWHAE-1) NBP2-79810

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Protocols

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