4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-23518

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0014 expressed in HEK293

Format

Azide and BSA Free
View all 4EBP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to residues around (Thr37, 46) of Human phospho-4EBP1

Modification

p Thr46, p Thr37

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.

Scientific Data Images for 4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free

4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free

Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] -

Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min;+) (line B); treated with IGF1 and -phosphatase (line C) using NBP3-23518 at 1:5000 dilution.
4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free

Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] -

Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from HEK293, untreated(line A) or treated with IGF1 (100ng/ml, 15min; +)(line B), using NBP3-23518 at 1:5000 dilution (upper) or Mouse Monoclonal 4EBP1 Antibody at 1:5000 dilution (lower).

Applications for 4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 4EBP1

4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5' cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)

Long Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Alternate Names

EIF4EBP1, PHAS-I

Gene Symbol

EIF4EBP1

Additional 4EBP1 Products

Product Documents for 4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 4EBP1 [p Thr46, p Thr37] Antibody (0014) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons

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