5-MethylCytosine Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-42813

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Immunoprecipitation, Methylated DNA Immunoprecipitation, Dot Blot, Electron Microscopy

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 5-MethylCytosine Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to 5-MethylCytosine / 5-mC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-MethylCytosine Antibody - BSA Free

Dot Blot: 5-MethylCytosine Antibody [NBP2-42813] - Analysis of anti-5-mC antibody with the synthetic DNA controls. DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody at a dilution of 1:4000. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine.

Applications for 5-MethylCytosine Antibody - BSA Free

Application
Recommended Usage

Dot Blot

1:100 - 1:2000

Electron Microscopy

1:10 - 1:500

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 5-MethylCytosine

Alternate Names

5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)

Additional 5-MethylCytosine Products

Product Documents for 5-MethylCytosine Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 5-MethylCytosine Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5-MethylCytosine Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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