Actin Antibody (25E3) - BSA Free

Novus Biologicals | Catalog # NBP3-26527

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 25E3 expressed in HEK293

Format

BSA Free
View all Actin Primary Antibodies »

Product Specifications

Immunogen

Synthesized peptide [UniProt P68133]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Actin Antibody (25E3) - BSA Free

Actin Antibody (25E3)

Immunohistochemistry: Actin Antibody (25E3) [NBP3-26527] -

Immunohistochemistry: Actin Antibody (25E3) [NBP3-26527] - Image of Actin Antibody (25E3) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Actin Antibody (25E3)

Western Blot: Actin Antibody (25E3) [NBP3-26527] -

Western Blot: Actin Antibody (25E3) [NBP3-26527] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissue.
All lanes: Actin Antibody at 0.95ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 42 KDa
Observed band size: 42 KDa
Actin Antibody (25E3)

Immunocytochemistry/Immunofluorescence: Actin Antibody (25E3) [NBP3-26527] -

Immunocytochemistry/Immunofluorescence: Actin Antibody (25E3) [NBP3-26527] - Staining of HepG2 cells with Actin Antibody (25E3) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
Actin Antibody (25E3)

Flow Cytometry: Actin Antibody (25E3) [NBP3-26527] -

Flow Cytometry: Actin Antibody (25E3) [NBP3-26527] - Overlay histogram showing Hela cells stained with Actin Antibody (25E3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Actin Antibody (25E3) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:30-1:200

Immunohistochemistry

1:50-1:500

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Actin

Actin is a 43kDa filament protien that is ubiquitously expressed by all eukaryotic cells. This wide expression makes Actin an ideal "housekeeping gene", which may even account for up to 50% of total cellular protein. Actin microfilaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Actin antibodies are most commonly used as controls for protein standardization or in cytoskeletan or cell cycle research.

Alternate Names

ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3

Gene Symbol

ACTA1

Additional Actin Products

Product Documents for Actin Antibody (25E3) - BSA Free

Certificate of Analysis

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Product Specific Notices for Actin Antibody (25E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Actin Antibody (25E3) - BSA Free

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  • Q: I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?

    A:

    I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

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