AG-2/AGR2 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-27392
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Scientific Data Images for AG-2/AGR2 Antibody - Azide and BSA Free
Western Blot: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]
Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - AGR2 expression in cancer cell lines of the biliary tract. AGR2 mRNA levels in six biliary tract cancer cell lines were measured by real-time RT-PCR. The AGR2 transcript levels were normalized against those of the GAPDH. Results shown are means +/- standard deviations of (1/2)CT of target - CT of GAPDH of three experiments. B. AGR2 protein expression was detected by western blot analysis with beta-actin as a loading control. The result shown is a representative of three experiments. Abbreviations: CBD, common bile duct; GB, gall bladder; AoV, ampulla of Vater; IHD, intrahepatic duct; HDB, hepatic duct bifurcation; WD, well differentiated; MD, moderately differentiated; PD, poorly differentiated [24]. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-804), licensed under a CC-BY license. Image using the standard format of this product.Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392]
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Formalin-fixed, paraffin-embedded human adenocarcinoma of the breast stained with AGR2 antibody (2 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Western Blot: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]
Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Analysis of AGR2 using AGR2 antibody. MCF7 (A), Caco-2 (B) and human stomach lysate (C) probed with AGR2 antibody at 1:100. were used for this test. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.Western Blot: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]
Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Alterations of tumor-associated phenotypes in AG-2-silenced SNU-478 cells. AG-2 protein expression in SNU-478:VEC and SNU-478:KDs was detected by western blot analysis with beta-actin as a loading control. The result shown is a representative of three experiments. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-804), licensed under a CC-BY license. Image using the standard format of this product.Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392]
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Staining of AGR2 using AGR2 antibody. Formalin-fixed, paraffin-embedded normal colon tissue probed with AGR2 antibody at 1:100 dilution.Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392]
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Formalin-fixed, paraffin-embedded human adenocarcinoma of the colon stained with AGR2 antibody (2-5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Simple Western: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]
Simple Western: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Simple Western lane view shows a specific band for AGR2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for AG-2/AGR2 Antibody - Azide and BSA Free
Immunohistochemistry-Frozen
Immunohistochemistry-Paraffin
Immunoprecipitation
Simple Western
Western Blot
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 27 kDa.
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Background: AG-2/AGR2
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Additional AG-2/AGR2 Products
Product Documents for AG-2/AGR2 Antibody - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for AG-2/AGR2 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars