AIP/ARA9 Antibody (PSH03-17)

Novus Biologicals | Catalog # NBP3-32012

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH03-17 expressed in HEK293
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Product Specifications

Immunogen

Synthetic peptide within human AIP/ARA9 aa 1-330 / 330. (Uniprot: O00170)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AIP/ARA9 Antibody (PSH03-17)

AIP/ARA9 Antibody (PSH03-17) Western Blot: AIP/ARA9 Antibody (PSH03-17) [NBP3-32012]

Western Blot: AIP/ARA9 Antibody (PSH03-17) [NBP3-32012]

Western blot analysis on different lysates with NBP3-32012 at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2:Jurkat cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: THP-1 cell lysate (20 µg/Lane) Lane 5: HCT 116 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: RAW264.7 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Lane 11: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 35 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32012 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for AIP/ARA9 Antibody (PSH03-17)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AIP/ARA9

Aryl-hydrocarbon receptor-interacting protein immunophilin homolog ARA9, also known as AIP and XAP-2, is known to affect the potency and efficacy of AHR agonists and have functional consequences on AHR signal transduction. ARA9 may also play a positive role in AHR-mediated signaling, by influencing its ligand receptivity and/or nuclear targeting ability. Defects in AIP can lead to familial isolated pituitary adenoma (FIPA), growth hormone-secreting pituitary adenoma (GHSPA), ACTH-secreting pituitary adenoma (ASPA), and prolactin-secreting pituitary adenoma (PSPA).

Alternate Names

ARA9, FKBP16, FKBP37, SMTPHN, XAP2, XAP-2

Gene Symbol

AIP

Additional AIP/ARA9 Products

Product Documents for AIP/ARA9 Antibody (PSH03-17)

Certificate of Analysis

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Product Specific Notices for AIP/ARA9 Antibody (PSH03-17)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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