alcohol dehydrogenase 4 Antibody (24GB5120)

Novus Biologicals | Catalog # NBP3-50786

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB5120
View all alcohol dehydrogenase 4 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human alcohol dehydrogenase 4 (Uniprot: P08319)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alcohol dehydrogenase 4 Antibody (24GB5120)

alcohol dehydrogenase 4 Antibody (24GB5120)

Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 4 Antibody (24GB5120)[NBP3-50786] -

Immunocytochemical staining of HepG2 cells with alcohol dehydrogenase 4 antibody (1:1,000). Nuclei were stained blue with DAPI; alcohol dehydrogenase 4 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
alcohol dehydrogenase 4 Antibody (24GB5120)

Flow Cytometry: alcohol dehydrogenase 4 Antibody (24GB5120)[NBP3-50786] -

Flow cytometric analysis of alcohol dehydrogenase 4expression in HepG2 cells using alcohol dehydrogenase 4 antibody (1:2,000). Green, isotype control; red, ADH4.
alcohol dehydrogenase 4 Antibody (24GB5120)

Western Blot: alcohol dehydrogenase 4 Antibody (24GB5120)[NBP3-50786] -

Western blotting analysis using alcohol dehydrogenase 4 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for alcohol dehydrogenase 4 Antibody (24GB5120)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase 4

alcohol dehydrogenase 4 encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

Alternate Names

ADH-2, alcohol dehydrogenase 4, alcohol dehydrogenase 4 (class II), pi polypeptide, Alcohol dehydrogenase class II pi chain, aldehyde reductase, EC 1.1.1, EC 1.1.1.1

Gene Symbol

ADH4

Additional alcohol dehydrogenase 4 Products

Product Documents for alcohol dehydrogenase 4 Antibody (24GB5120)

Certificate of Analysis

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Product Specific Notices for alcohol dehydrogenase 4 Antibody (24GB5120)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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