ALDH6A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82634

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ALDH6A1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NNNPYGNGTAIFTTNGATARKYAHLVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSSPAVVMP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH6A1 Antibody - BSA Free

Western Blot: ALDH6A1 Antibody [NBP1-82634]

Western Blot: ALDH6A1 Antibody [NBP1-82634]

Western Blot: ALDH6A1 Antibody [NBP1-82634] - Analysis using Anti-ALDH6A1 antibody NBP1-82634 (A) shows similar pattern to independent antibody NBP1-82635 (B).
Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634] - Staining of human testis.
Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634] - Staining of human liver shows strong cytoplasmic positivity with a granular pattern in hepatocytes.
Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634] - Staining of human tonsil shows low expression as expected.
Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634] - Staining in human liver and tonsil tissues using anti-ALDH6A1 antibody. Corresponding ALDH6A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634] - Staining of human cerebral cortex, kidney, liver and testis using Anti-ALDH6A1 antibody NBP1-82634 (A) shows similar protein distribution across tissues to independent antibody NBP1-82637 (B).
Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634] - Staining of human kidney.
Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634]

Immunohistochemistry-Paraffin: ALDH6A1 Antibody [NBP1-82634] - Staining of human cerebral cortex.
ALDH6A1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: ALDH6A1 Antibody [NBP1-82634]

Immunocytochemistry/ Immunofluorescence: ALDH6A1 Antibody [NBP1-82634]

Staining of human cell line MCF7 shows localization to mitochondria.

Applications for ALDH6A1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH6A1

ALDH6A1 belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.

Alternate Names

aldehyde dehydrogenase 6 family, member A1, Aldehyde dehydrogenase family 6 member A1, EC 1.2.1.18, EC 1.2.1.27, malonate-semialdehyde dehydrogenase, Malonate-semialdehyde dehydrogenase [acylating], methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial, MGC40271, MMSADHA, MMSDHmitochondrial acylating methylmalonate-semialdehyde dehydrogenase

Entrez Gene IDs

4329 (Human)

Gene Symbol

ALDH6A1

UniProt

Q02252 (Human)

Additional ALDH6A1 Products

Product Documents for ALDH6A1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ALDH6A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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